摘要
目的 :在背根节 (DRG)慢性压迫模型上 ,采用单纤维记录神经元自发放电和生化检测受损组织中蛋白激酶A(proteinkinase ,PKA)活性的方法 ,研究PKA在DRG压迫损伤后感觉神经元中自发放电的作用。结果 :压迫损伤侧DRG组织中的PKA磷酸化PepTag肽百分数为 2 5 .5 1± 2 .6 2 % ,较未受压迫侧和正常组DRG组织明显增加 (P <0 .0 5 )。PKA催化亚单位抑制剂H 89(1 0 μM )可以明显抑制受损DRG神经元的自发放电 ,抑制百分数为 76 .91± 1 3.79%。结论 :受损DRG组织中PKA活性上调 ,高活性的PKA参与介导受损DRG神经元的自发放电。
Aim: To study the role of protein kinase A (PKA) in the hyperexcitability of injured neurons in the compression of dorsal root ganglion (DRG) model, spontaneous activities and PKA activities in injured neurons were recorded and tested by the method of single fiber recording and biochemical assay respectively. Results: The percentage of PKA phosphorated PepTag peptide in compressed DRGs was 25.51±2.62%, which was significantly enhanced compared with those in uncompressed and normal DRGs. An inhibitor of PKA catalytic subunit H 89 (10μM) suppressed the spontaneous activity of injured DRG neurons obviously. The suppressive percentage was 76.91±13.79%. Conclusion: PKA activity in injured DRGs was enhanced, and highly activated PKA contributed to the mediation of the spontaneous activity from injured DRG neurons.
出处
《中国疼痛医学杂志》
CAS
CSCD
2000年第4期229-234,共6页
Chinese Journal of Pain Medicine
基金
国家自然科学基金!提供资助 (No .39830 16和 39970 2 4 2 )
关键词
蛋白激酶A
自发放电
背根节神经元受损
Protein kinase A
Spontaneous activity
Dorsal root ganglion
Afferent neuro
