“肺与大肠相表里”--肺与大肠功能之间的交互作用

Experimental Study on Functional Connection between the Lung and Large Intestine Meridians in the Rat:Cross-talk between the Lung and Large Intestine Meridians

目的:探讨肺经经脉、大肠经经脉与直结肠相关联系的特异性,阐述肺与大肠相表里的科学内涵。方法:40只SD麻醉大鼠,分离出左交感神经节后心肺支。以铂金丝电极记录心肺交感神经放电,用水囊接压力换能器-放大器记录直-结肠内压力(运动)变化;内脏痛采用直-结肠气囊扩张刺激的方法。分别电针刺激(6mA)肺经"太渊-经渠"、大肠经"阳溪-偏历",观察对肺心交感神经放电和直结肠运动的影响;电针"上巨虚-足三里"观察对直结肠运动的作用;给予直结肠扩张刺激(60mmHg、80mmHg),观察对电针诱发的心肺交感神经活动的影响。结果:心肺交感神经一般没有自发活动,6mA电针分别刺激肺经经脉"太渊-经渠"、大肠经经脉"阳溪-偏历"诱发心肺交感神经(11.26±2.34)个放电(n=10)、(10.73±2.62)个放电(n=11);随着刺激强度的增加,心肺交感神经诱发反应也随之加大,两条经穴位引起的反应并无明显差别(P>0.05)。电针肺经经脉"太渊-经渠"、大肠经经脉"阳溪-偏历"和大肠经下合穴"上巨虚-足三里"均使直结肠的运动幅值显著提高(P<0.05)。60mmHg直结肠扩张刺激抑制了电针肺经经脉"太渊-经渠"对肺心交感活动的激活作用,抑制率为43.46%;增加到80mmHg时,抑制率达到67.42%。两种强度的直结肠扩张刺激对电针作用的影响差异有统计学意义(P<0.01)。结论:刺激肺经经脉与大肠经经脉对心肺交感神经有激活作用,对直结肠运动有促进作用;直结肠伤害性扩张刺激能抑制电针肺经经脉对心肺交感神经的激活作用。说明肺和直结肠存在相互对应的联系。

Objective According to the theory of traditional Chinese medicine about ＂close association between the lung and the large intestine＂, the present study was designed to investigate the functional relationship between the Lung and Large In- testine Meridians and the colorectum by using electrophysiological methods. Methods A total of 40 SD rats were used in the present study. Under anesthesia, the cardiopulmonary branch of the left sympathetic nerve （CPSN） was separated for recording the electrical activities with a pair of platinum wire electrodes. Intra-colorectal pressure was recorded using a water-balloon in- serted into the anus and connected to a pressure-transducer for observing rectocolonic pressure changes （mobility）. Electroacu- puncture （EA） stimulation was applied to bilateral ＂Taiyuan＂ （LU 9）- ＂Jingqu＂ （LU 8） of the Lung Meridian, ＂Yangxi＂ （LI 5）- ＂Pianti＂ （LI 6） of the Large intestine Meridian, and ＂Shangjuxu＂ （ST 37）-＂Zusanli＂ （ST 36） of the Stomach Meridian, respective- ly. Colorectal distension （CRD, visceral pain stimulation, 60 mmHg and 80 mmHg） was performed using an air-balloon for obse- rving its influence on EA stimulation of LU 9- LU 8 induced changes of electrical activities of CPSN. Results Under anesthesia conditions, no spontaneous electrical activities of the CPSN were found in rats. After EA stimulation （3 times of the threshold in- tensity） of the bilateral ＂Taiyuan＂ （LU 9）- ＂Jingqu＂ （LU 8） of the Lung Meridian, and ＂Yangxi＂ （LI 5）-＂Pianli＂ （LI 6） of the Large Intestine Meridian, the evoked discharge numbers of the cardiopulmonary sympathetic nerve were （11.26-＋ 2.34） and （10. 73 4- 2.62） spikes, respectively, without significant differences between the two groups （P^0.05）. Compared with pre-stimulation, the colorectal motility （amplitudes of the intra-colorectal pressure waves） was obviously increased following EA stimulation of LU ^-LU 8 of the Lung Meridian, LI 5-LI 6 of the Large Intestine Meridian and ＂Shangjuxu＂ （ST 37）-＂Zusanli＂ （ST 36） , Lower-Ho（Sea） acupoints of the Large Intestine Meridian （P〈0.05）. After CRD stimulation at 60 mmHg and 80 mmHg, the LU 9- LU 8- stimulation evoked discharge rates of OPSN were reduced by 43.46% and 67.42%, respectively. Conclusion EA stimulation of LU 9- LU 8 of the Lung Meridian and LI 5-LI 6 of the Large Intestine Meridian can activate discharges of CPSN, and increase the colorectal motility in the rat. ORD stimulation inhibits the activated effect of EA on electrical activities of OPSN. These results show a functional communication or cross-talk between the lung and the colorectum.