电针对多囊卵巢综合征大鼠卵巢高雄激素症的影响

Effects of Electroacupuncture of“Guanyuan”(CV 4)-“Zhongji”(CV 3)on Ovarian P 450arom and P 450c17αExpression and Relevant Sex Hormone Levels in Rats with Polycystic Ovary Syndrome

目的:观察电针对来曲唑诱导的多囊卵巢综合征(PCOS)大鼠卵巢P 450芳香化酶(arom)和P450 17α羟化酶(c 17α)表达及相关性激素的影响。方法:将30只雌性SD大鼠随机分成正常组、模型组、电针组各10只,模型组和电针组采用来曲唑灌胃复制PCOS模型。电针组选取"关元""中极"给予电针治疗,每日1次,治疗14d。观察大鼠卵巢质量、卵巢组织形态学及超微结构变化;免疫组化法观察卵泡颗粒细胞层P 450arom和卵泡膜细胞层P 450c17α的表达;ELISA法检测卵巢组织相关性激素雌二醇(E 2)、雌酮(E 1)、雄烯二酮(ASD)、睾酮(T)、促卵泡刺激素(FSH)、促黄体生成素(LH)含量变化。结果:与正常组相比,模型组大鼠左、右卵巢组织质量均有显著增加(P<0.01),电针组较模型组则显著降低(P<0.01);同时模型组大鼠卵巢组织形态学及超微结构也出现异常改变,而经电针治疗后大鼠卵巢组织形态学及超微结构均有明显改善。与正常组比较,模型组大鼠卵巢颗粒细胞层P 450arom表达降低(P<0.01),卵泡膜细胞层P 450c17α表达显著增高(P<0.01);电针组P 450arom表达较模型组增高(P<0.05),P 450c17α表达则降低(P<0.01)。与正常组比较,模型组大鼠卵巢组织ASD、T、LH水平显著升高(P<0.01),E 1、E 2水平显著降低(P<0.01);而电针组大鼠ASD、T、LH水平较模型组显著降低(P<0.05,P<0.01),E 1、E 2水平显著升高(P<0.01)。结论:电针可通过促进/增加PCOS大鼠卵巢颗粒细胞层P 450arom表达、抑制/减弱卵泡膜细胞层P 450c17α表达,促使卵巢内雄激素能向雌激素正常转化,由此改善PCOS卵巢局部内分泌环境紊乱,达到恢复或改善其卵泡发育及排卵异常的作用。

Objective To observe the effect of electroacupuncture （EA） on ovarian P 450 arom and P 450 c 17 a （aro- matases） expression and related sex hormone levels in polycystic ovary syndrome （PCOS） rats. Methods Thirty SD rats were randomly divided into normal control group, model group and EA group （10 rats/group）. PCOS model was made by intragastric administration of letrozole at 1 mg/kg per day for consecutive 21 days. ＂Guanyuan＂ （CV 4） and ＂Zhongji＂ （CV 3） acupoints were stimulated 20 min by EA （2 mA, 2 Hz）, once daily for consecutive 14 days. The damp ovarian weight was weighed and the patho- logical changes of the ovarian tissue were observed after H.E. staining. Ultrastructural changes of the ovarian tissue were ob- served by transmission electron microscope. Immunohistochemical staining was adopted to detect ovarian follicle granulosa cell P 450 arom and follicle membrane cell P 450 c 17 a expression. The contents of estradiol （E 2）, estrone （E 1）, androstenedione （ASD）, testosterone （T）, follicle-stimulating hormone （FSH） and luteinizing hormone （LH） in the ovarian tissue were measuredby ELISA. Results Compared with the normal group, there was a significant increase in the damp weight of both left and right ovarian tissues in the model group （P〈0.01）. After EA, the ovarian weight was remarkably reduced （P〈0.01）. Pathological changes of the ovarian tissue such as thickening of the superficial albugineous coat of the ovary, thinning of the granular cell layer, and disappearance of the intraovular oocytes and coronaradiata under light microscope, and mitochondrion swelling, fracture or disappearance of mitochondrial cristae, and enlargement of the endoplasmic reticulum, etc. after modeling were obviously im- proved in the EA group. In comparison to the control group, the expression of the follicle granulosa cell P 450 arom was significant- ly down-regulated and that of follicle membrane cell P 450 c 17 a was significantly upregulated in the model group （ P〈0.01 ）. Af- ter EA intervention these changes were obviously reversed （P〈0.05, P〈0.01 ）. In the model group, there was a significant in- crease in the levels of ASD, T and LH in the ovarian tissues （P〈0.01） and a marked decrease in the contents of ovarian E 1 and E2 （P〈0.01） in comparison to the control group. After EA, the ovarian ASD, T and LH levels were notably decreased （P〈0.05, P〈0.01 ） and the E 1 and E 2 levels apparently increased （P〈0.01） compared with the model group. Conclusion EA can improve letrozole-induced pathological changes of ovarian morphology and ultrastructure and obviously promote P 450 arom expression in the follicle granulosa cell layer and inhibit P 450 c 17 a expression in the follicle membrane cell layer, as well as re- gulate sex hormone levels in PCOS rats, facilitating the normal transformation of ovarian androgen to estrogen and restoring the lo- cal endocrine disorders.