针刺“内关”“神门”穴对高脂血症大鼠心肌梗死后室旁核区和血清5-羟色胺含量的影响

Effects of Electroacupuncture Stimulation of“Neiguan”(PC 6)and“Shenmen”(HT 7)on Con-tents of 5-hydroxytryptamine in Hypothalamic Paraventricular Nucleus Region and Serum in Hy-perlipidemia Rats with Acute Myocardial Infarction

目的:观察针刺"内关""神门"穴对高脂血症大鼠急性心肌梗死(AMI)后室旁核(PVN)区和血清5-羟色胺(5-HT)含量的影响。方法:从80只高脂血症SD大鼠中随机选择20只作为假手术组,其余大鼠结扎冠状动脉左前降支以复制AMI模型。将复制成功的大鼠随机分为模型组、电针内关组、电针神门组,每组20只。两个电针组大鼠分别接受双侧"内关"穴、"神门"穴电针治疗,每日1次,共5d。分别采用酶联免疫吸附法和放射免疫分析法测定PVN区和血清5-HT的含量。结果:心肌梗死后的高脂血症大鼠下丘脑PVN区5-HT含量显著降低,而血清中5-HT含量显著增多(P<0.01);在电针"神门"穴或"内关"穴后,5-HT含量相应地在PVN中增多,而在血清中减少(P<0.01)。结论:在高脂血症大鼠AMI期,中枢对外周交感神经兴奋的调控可以通过释放5-HT实现,而电针"神门"穴或"内关"穴可以通过影响中枢分泌5-HT进而实现对AMI的治疗作用。同时,心经经脉、心包经经脉与心脏、下丘脑之间的生理学联系中不具有相对特异性。

Objective To observe the effects of electroacupuncture （EA） stimulation of ＂Neiguan＇（PC 6） and ＂Shen- men＂（HT 7） on 5-hydroxytryptamine （5-HT） levels in the paraventricular nucleus （PVN） region of hypothalamus and serum in hy- perlipidemia rats with acute myocardial infarction （AMI）. Methods A total of 80 SD rats with hyperlipidemia were used in the present study. Among them, 20 rats were randomly selected to be the sham-operation group, and the rest 60 cases were used to make the AMI model by occlusion of the anterior descending branch of the left coronary artery. The 60 rats who were identified to be successful in AMI were randomly divided into model group, EA-PO 6 group and EA-HT 7 group （20 rats/group）. EA （2 Hz, 2 mA） was applied to bilateral PC 6 and HT 7 for 20 min, once daily for 5 days. The contents of 5-HT of hypothalamic PVN region and serum were examined by enzyme linked immunosorbent assay （ELISA） and radioimmunoassay, respectively. Results Fol- lowing AMI, marked myocardial injury （swelling, breakage, disappearance of local transverse striations and necrosis of myo- cytes） with infiltration of neutrophil granulocytes was found in the model group, while in both EA-PO 6 group and EA-HT 7 group, moderate swelling （no breakage） of fewer myocardial fibers with mild infiltration of neutrophil granulocytes was found, indicating a marked improvement of myocardial injury after EA treatment. Compared with the sham-operation group, the content of 5-HT was obviously decreased in the hypothalamic PVN region and significantly increased in serum in the model group （P^0.01）. After EA intervention, the content of 5-HT was considerably up-regulated in the hypothalamic PVN region and apparently down-regulated in serum in both EA-PO 6 and EA-HT 7 groups in comparison with the model group （P^0.01）. No significant differences were found between EA-PC 6 and EA-HT 7 groups in regulating hypothalamic and serum 5-HT levels （ P~〉0.05）. Conclusion EA stimula- tion of both PC 6 and HT 7 can relieve acute ischemic injury of the myocardium in hyperlipidemia rats, which is probably partially related to its effect in promoting the release of 5-HT from the hypothalamic PVN region.