急性胎兔宫内窘迫模型脑组织病理及NMDAr1表达实验研究

Experimental study on NMDAr1 expression and pathology research on brain tissue of rabbits acute fetal distress model

目的建立孕兔宫内窘迫模型,观察胎兔脑组织病理及NMDAr1表达情况。方法新西兰大白兔孕兔30只,随机分为缺血模型组(n=9)、假手术组(n=9)和空白组(n=12)。缺血模型组孕兔行开腹子宫动脉结扎术,建立急性胎兔宫内窘迫模型;假手术组行开腹术,不结扎子宫动脉;空白组不行任何手术处理。除空白组胎兔直接断头取脑外,其他组胎兔都在术后6h断头取脑,观察各组胎兔脑组织病理形态及NMDAr1表达情况。结果与空白组及假手术组比较,缺血模型组胎兔脑组织NMDAr1表达呈强阳性,病理形态学为明显的脑组织细胞损伤表现;空白组和假手术组胎兔脑组织NMDAr1表达基本相同,均为少量表达,病理形态学均无脑组织细胞损伤表现。结论急性宫内窘迫可致胎兔脑组织NMDAr1表达增加,可能是造成其脑组织病理损伤的重要机制。

Objective Building rabbits acute fetal distress model to observe NMDAr1 expression and pathology research on brain tissue. Methods 30 Zelanian pregnant rabbits were divided into model group（n=9）, sham group（n=9） and blank group（n=12）randomly. Rabbits of model group were performed and Uterine artery ligation to build acute fetal distress model. Rabbits of sham group were just performed laparotomy, and blank group did not receive any operation. Except the blank group＇s fetal rabbits were beheaded and take the brain directly, other groups＇s fetal rabbits were beheaded and take the brain after 6 hours of postoperation. Then, observe NMDAr1 expression and pathology research on brain tissue. Results 3 groups were compared multiply. In model group, the NMDAr1 expression of the fetal rabbits were strong positive, and pathomorphism significantly showed brain tissue and cell injury. Blank group＇ result is similar to sham group＇s, which were different from model group＇s. The NMDAr1 expression of two group＇s fetal rabbits were little positive, and pathomorphism did not show brain tissue and cell injury. Conclusion Acute fetal distress can cause the fetal rabbits＇s NMDAr1 expression higher, that may be a critical mechanism causing the brain pathological injury.