肝型碱性磷酸酶测定方法对原发性肝癌的诊断价值

Assaying method of liver-type al kaline phosphatase to primary hepatocarcinoma

为探讨用麦胚凝集素 (WGA)和蔓陀萝凝集素 (DSA )亲和层析分离血清中的肝型碱性磷酸酶 (L -AL P)和骨型碱性磷酸酶 (B- AL P)诊断原发性肝癌的临床价值 ,分别对正常人、慢性肝病、原发性肝癌患者的血清进行了检测。结果显示 ,正常人血清 L- AL P不与 DSA结合 ,慢性肝炎及肝硬化患者分离到 DSA弱结合组分 ,原发性肝癌出现了 DSA强结合组分 ,5 8例原发性肝癌患者血清中阳性出现率为 93.1%。提示 L- AL P的 DSA强结合组分为原发性肝癌特异性标志 ,L- AL P凝集素亲和层析是一种诊断原发性肝癌灵敏可靠的方法。

To study the diagnosis value of WGA(wheat germ agglutinin)and DSA(darura stramonium agglutinin)lectin affinity chromatography separating serum liver type alkaline phosphatase (L ALP)and bone type alkaline phosphatase(B ALP)to primary hepatocarcinoma The serum of nomal persons,patients with chronic liver diseases and primary hepatocarcinoma was detected.Results showed that L ALP in normal serum couldn′t bind to DSA,the weakly binding fraction of L ALP was present in chronic liver diseases and primary hepatocarcinoma,the strongly binding fraction of L ALP had been found only in primary hepatocarcinoma and positive rate was 93 1% in 58 patients with hepatocarcinoma The strongly binding fraction of L ALP is a specific marker of primary hepatocarcinoma,lectin affinity chromatography of L ALP is a sensitive and reliable method for diagnosis of primary hepatocarcinoma