可溶性E-和P-选择素与急性脑梗死的关系

Soluble E- and P-selectins in acute cerebral infarction

目的 探讨E -和P -选择素与脑梗死的关系。方法 用ELISA方法测定 30例脑梗死、2 5例脑出血患者及2 8例正常人的sE -和sP -选择素血清水平 ,分析它们与WBC、N %、TNF及IL - 6血清水平之间的关系。结果 急性期脑梗死患者sE -和sP -选择素血清水平 [分别为 (6 5 2± 39 4)ng/ml、(399± 134)ng/ml]明显高于脑出血患者 [分别为 (4 4 6± 11 8)ng/ml、(2 43± 112 )ng/ml]及正常人 [分别为 (4 0 5± 12 5 )ng/ml、(2 2 7± 10 6 )ng/ml]。而该组患者恢复期这两种选择素水平无明显升高。急性期脑梗死患者sE -和sP -选择素水平分别与WBC、N %、IL - 6水平呈正相关关系 ,sP -选择素水平还与TNF水平呈正相关关系。结论 sE -和sP -选择素血清水平增高反映脑梗死急性期E -和P -选择素可能在TNF和IL - 6的刺激下 。

Objective To determine the roles of E-and P-selectins in the pathogenesis of cerebral infarction.Methods The serum concentrations of sE-and sP-selectins were measured with enzyme-linked immunosorbent assay (ELISA)in 30 cerebral infarction、25 cerebral haemorrhage and 28 healthy control subjects.The correlations between selectins and WBC、N%、the serum level of TNF or the serum level of IL-6 were analyzed.Results The serum levels of sE- and sP- selectins in acute stage of cerebral infarction group[(65 2±39 4)ng/ml,(399±134) ng/ml,respectively]were significantly higher than those in acute stage of cerebral haemorrhage[(44 6±11 8)ng/ml,(243±112) ng/ml,respectively] and those in healthy control group[(40 5±12 5)ng/ml,(227±106) ng/ml,respectively].In acute stage of cerebral infarction,the serum concentration of sE- selectin, as well as the level of sP-selectin,was observed in positive correlation with the WBC、N%、or the level of IL-6 respectively.The level of sP- selectin was also positively correlated with the level of TNF.Conclusions The increased levels of sE- and sP- selectins reflected that these two selectins might be enhanced the endotheliocyte-leukocyte adhesion,under the stimulations of TNF and IL-6,so as to be involved in the cerebral injury after acute cerebral infarction.