摘要
目的观察小胶质细胞在激光诱导小鼠脉络膜新生血管(CNV)发生过程中的表达特点及意义。方法雄性CX3CR1+/GFP杂合性转基因小鼠32只,取每只小鼠一只眼作为实验组,予532nm激光光凝,激光功率50兆瓦,曝光时间100ms,光斑直径100μm,每眼光凝4点,另一只眼作为对照组,在光凝后分别于7d、14d、21d、28d按随机数字法选取8只小鼠处死,摘出眼球,制作病理标本,用荧光免疫组化染色方法检测CD11b、Iba1的表达,并用激光共聚焦显微镜进行观察。结果光斑处实验组较对照组小胶质细胞增生明显(P<0.05),并且14d组及21d组小胶质细胞较7d组增生明显,在细胞数量、细胞面积、平均光密度值等方面的差异均具有统计学意义(P<0.05),28d组小胶质细胞增生程度下降,与7d组接近。结论小胶质细胞在激光诱导CNV模型中激活,随着损伤时间推移先升高,后降低,在CNV的形成过程中发挥重要作用。
Objective To observe the characteristics and significance of microglia in the process of mice choroid neo- vascularization induced by laser. Methods Selected 32 male CX3CRlVaW heterozygous transgenic mice. One eye of every mouse received a series of 4 spots of laser irradiation （532nm,50MV, 100p^m, 100ms）as the experimental group, another eye as control group. 8 mice were killed randomly after photocoagulation 7d,14d,21d,28d. The eyeballs were enucleated and made pathological specimens. Immunofluorescence staining method was used to detect the expression of C D1 l b,Ibal,and was ob- served by confocal laser scanning microscopy. Results Compared with control group,microglial cell had higher hyperplasia lever（P〈0.05）. Compared with 7d experimental group,there were significant difference in the aspect of the number and the aver- age optical density value for 14d group and 21 day group（P〈0.0.5）,but not 28d group. Conclusion Microglia cells were acti- vated in CNV model induced by laser, and increased first,then decreased,which played an important role in the formation of CNV.
出处
《中国现代医药杂志》
2013年第11期5-7,共3页
Modern Medicine Journal of China
