摘要
目的 :在考察淫羊藿苷 (icariin)于人工胃液中稳定性的基础上 ,研究了肠道内细菌对淫羊藿苷的代谢作用。方法 :于人工胃液或肠内菌培养液中 ,加入淫羊藿苷温孵培养一定时间后 ,以薄板层析、高效液相色谱仪和电喷雾质谱仪 ,对培养物成分做定性分析检查。结果 :淫羊藿苷在人工胃液中有较高的稳定性。离体培养人肠道内细菌可代谢淫羊藿苷 ,且其主要代谢产物为淫羊藿苷的苷元 (icaritin)及其苷元的异戊烯基位置异构体。在大鼠整体实验中 ,从粪便和尿液中均检出一主要代谢产物 ,并初步确定此代谢产物为宝藿苷 (baoliuoside )。结论 :在离体条件下 ,淫羊藿苷可被人肠内菌代谢 ,主要代谢产物为淫羊藿苷的苷元。大鼠灌服淫羊藿苷后 ,吸收入血的主要代谢物为宝藿苷 。
The stability of icariin in artificial gastric juice and the transformation of icariin in intestine were studied Icariin was incubated with artificial gastric juice or intestinal flora for a certain period of time, and its metabolite examined by TLC, HPLC and ESI/MS Results showed that icariin was stable in artificial gastric juice, but was metabolized to icaritin by human intestinal bacteria in vitro A main metabolite, baohuoside Ⅰ was found in urine and feces of rat after ig administration of 200 mg/kg icariin These results showed that icariin can be metabolized in vitro by human intestinal bacteria to icaritin and as baohuoside after oral administration
出处
《中草药》
CAS
CSCD
北大核心
2000年第11期834-837,共4页
Chinese Traditional and Herbal Drugs
基金
国家中医药管理局资助
