肝纤维化大鼠肝组织MAPKs信号通路的活化及意义

Activation of Liver MAPKs Signaling Pathway in Rats with Liver Fibrosis and Its Significance

目的:观察CCl4诱导的肝纤维化大鼠肝组织MAPKs信号通路的ERK通路、JNK通路、P38MAPK信号通路的变化,探讨其作用和意义。方法:采用40%CCl4皮下注射建立大鼠肝纤维化模型,采用实时荧光定量PCR技术检测肝组织MAPKs信号通路的关键信号分子ERK1、JNK2、P38基因的mRNA表达;采用Western blot技术检测大鼠肝组织ERK1/2、JNK2、P38的总蛋白表达及磷酸化水平。结果:模型大鼠肝组织ERK1、JNK2、P38基因的mRNA表达较正常组明显增强,磷酸化的ERK1/2、JNK2、P38蛋白水平也较正常组显著增加。结论:肝纤维化大鼠肝组织MAPK信号途径中的ERK、JNK及p38MAPK三条信号通路均被激活,参与了肝纤维化的进展。

Objective ：To observe the change of ERK signaling pathway, JNK signaling pathway and P38MAPK signa- ling pathway of liver MAPKs signaling pathway in rats with liver fibrosis induced by CC14 , and to explore the effect and significance. Methods ： The model with liver fibrosis was induced by intraperitoneal injection 40% CCI4. The gene mRNA of ERK1 ,JNK2 and P38 ,which were key signaling molecules of MAPKs signaling pathway,were detected with RT - PCR; the total protein expressions and the phosphorylation levels of ERK1 ,JNK2 and P38 were detected with Western blot. Re- sults： The mRNA expression of hepatic ERK1 ,JNK2 and P38 in models were obviously enhanced than those in the normal group. And the phosphorylation protein expressions of ERK1/2 ,JNK2 and P38 were also significantly enhanced than that in the normal group. Conclusion： ERK,JNK and p38MAPK signaling pathways of liver MAPKs signaling pathway in rats with liver fibrosis were all activated, involving in the progression of liver fibrosis.