肝硬化患者血清肿瘤坏死因子-α、白细胞介素-8、β_2-微球蛋白相关性的研究

Study of correlation between serum TNF-α、IL-8 and β_2-MG in patients with cirrhosis

目的 探讨肝硬化患者血清TNF α、IL 8和 β2 MG的关系及意义。方法 用ELISA双抗体夹心法及放射免疫法检测了 30例肝硬化患者及 30例正常人血清中TNF α、IL 8和 β2 MG的浓度。结果 肝硬化患者血清中TNF α、IL 8和 β2 MG明显升高。TNF α、IL 8与血清GPT呈正相关 ,提示TNF α、IL 8在肝硬化时参与了肝细胞损害过程 ;β2-MG与血清GPT呈正相关 ,提示 β2 MG可间接反映肝病活动程度 ;TNF α与IL 8呈正相关 ,提示TNF α诱导了IL 8的产生。结论 炎症因子TNF α、IL 8参与了肝细胞损害过程 ,β2 MG可间接反映肝病活动程度。

Objective To determine the relationship between serum TNF α,IL 8 and β 2 MG in patients with cirrhosis.Methods Serum TNF α,IL 8 and β 2 MG were detected in 30 patients with cirrhosis and 30 normal controls by ELISA and radioimmunoassay method. Results The patients with cirrhosis showed higher levels of serum TNF α,IL 8 and β 2 MG than normal controls. Both TNF α and IL 8 had positive correlations with GPT, which suggested that the two cytokines were associated with the process of liver damage.Serum β 2 MG had positive correlation with GPT,which suggested that serum β 2 MG may reflect indirectly the degree of liver disease activity.TNF α had positive correlation with IL 8, which suggested that TNF α resulted the product of IL 8 .Conclusion The inflammatory cytokines TNF α,IL 8 are involved in hepatocellular damage in cirrhosis. Serum β 2 MG may reflect indirectly the degree of liver disease activity.