左旋精氨酸对急性心肌梗塞大鼠选择素表达的影响

Effect of L-arginine on Expression of Seletins in Rats with Acute Myocardium Infarction

为探讨一氧化氮 (NO)的前体左旋精氨酸 (L -arg)对急性心肌梗塞 (AMI)大鼠心肌有无保护作用及其作用机制 ,采用垂体后叶素 (Pit)AMI模型。设立正常对照组、阳性对照组、及L -arg治疗组 ,观察中性粒细胞 (PMN)L -选择素及心肌微血管内皮细胞P -选择素的表达 ,并测定心肌梗塞范围、PMN浸润数及血浆NO、丙二醛 (MDA)的浓度。结果表明 :阳性对照组L -选择素及P-选择素表达上调 ,梗塞心肌PMN浸润数、血浆MDA的浓度明显升高 ,血浆NO的浓度降低。L-arg治疗组上述指标改变的程度明显减轻 (P <0 0 1) ,心肌梗塞范围缩小。提示外源性L -arg对AMI有防治意义 ,其作用机制与NO抑制选择素的表达有关。

The present study was to assess the efficacy of NO precursor——L-arginine on acute myocardium infarction(AMI)in rats. 30 Wistar rats were divided into three groups:Control group received a saline injection,AMI group was given an injection of 30 U/Kg of pit, L-arg group was injected with L-arg as soon as pit was give. As compared with AMI group, L-arg exerted marked attenuation of cardiac injury:reduction of infarct area and neutrophil infiltration and decrease of the concentration of MDA in plasma. The expression of L-selectin and P-selectin were lower than that in AMI group. The results suggest that L-arg exerts significant cardiproitective effects in AMI since there is a positive correlation between the cardioprotection of L-arg and the inhibition of NO on selectins expression.