不同细胞年龄的红细胞膜LP(A)含量与血浆含量的相关性

The Contents in RBC Membrane at Several Cell Ages and the Relationship with Its Level in Plasma

收集 96名门诊病人的肝素抗凝血标本 ,用抗低渗法将红细胞分为老、中、青三组 ,离心分离出不同细胞年龄的红细胞膜 ,测出膜上的LP(A)的含量。各组的LP(A)测定结果 (mg/ g膜蛋白 )为 :老龄组 70 2± 18 8、中龄组 60± 8 2、青龄组 34 0± 12 0。结果表明 :随细胞年龄的增加膜LP(A)含量有逐渐升高的趋势 ,老 -中、老 -青、中 -青组之间均有显著差异 (P <0 0 5) ,且膜LP(A)总含量 ( 54 53± 11 1)mg/ g膜蛋白与血浆中LP(A)含量 ( 190 2± 32 54)mg/L呈正相关 ,R =0 9617,P <0 0 1。膜LP(A)的沉积量取决于血浆中的LP(A)含量 。

Use the anti-low-osmosis for RBC to divede 96 outpatients to three groups of aging middle age and youth and separate with centrifugation to get RBC membrane at different cell ages and determining the contents of LP(a) in membrane.The contents(mg/g membrane protein)of LP(a) are (69.8±48.55) in aging group,(59.9±39.63) in middle age group and (4.0±12.0) in youth group respectively. The LP(a) content of membrane increase with the raise of level in plasma and there is a positive relationship between the LP(a) content of membrane and its level in plasma (R=0.961 7,P<0.05). The results suggest the sediment content in RBC membrane lie on the level in plasma and the integrality of membrane may be one of important factors for controlling the sediment content.