泡盛曲霉产菊粉酶发酵条件的探究

Fermentation conditions of Aspergillus awamori for inulinase production

由山东滨海盐碱地菊芋生长的根际土壤中分离得到高产菊粉酶的菌株,经形态学观察、18S rDNA全序列分析,初步鉴定该菌为泡盛曲霉(Aspergillus awamori SD1222)。采用单因素试验和正交试验对其进行了产酶条件优化,确定该菌株产菊粉酶的发酵条件为:菊粉浓度30g/L,蛋白胨浓度6g/L,氯化钠浓度7g/L,250mL锥形瓶中的装液量为40mL,初始pH值为5.0,30℃下160r/min振荡培养72h。

A strain with high inulinase productivity was screened from the seacoast of Shangdong province, and identified as Aspergillus awamori through observation by morphology and identification of 18S rDNA gene partial sequence. Based on the results of single factor experiments and or- thogonal experiments, the optimal fermentation levels of the main factors were as follow： inulin 30g/L, peptone 6g/L, NaCl 7g/L, volume of medium 40ml/250ml Erlenmeyer flask, initial pH 5.0, cultivation temperature 30℃, rotation speed 160r/min and fermentation time 72h.