摘要
目的 研究胆管癌中p5 3,p2 1WAF1基因表达以及它们之间的关系 ,以期为找到一种早期诊断及判断胆管癌预后的方法打下一个基础。方法 本研究采用 30例人胆管癌及癌旁组织配对资料 ,应用原位杂交及免疫组化方法检测p5 3 ,p2 1WAF1基因表达并探讨它们之间的关系 ,进而分析它们与临床病理资料之间的相关性。结果 原位杂交检测 30例胆管癌中有 14例 (14/30 ,46 7% )呈p5 3阳性 ,12例 (12 /30 ,40 0 % )p2 1WAF1阳性。p5 3,p2 1WAF1原位杂交结果与胆管癌的临床病理资料无明显相关性。免疫组化检测 30例胆管癌中 ,P5 3蛋白阳性 16例 (16 /30 ,5 3 3% ) ,P2 1WAF1阳性 11例 (11/30 ,36 7% )。有局部淋巴结转移组P5 3基因蛋白表达阳性率明显高于无局部淋巴结转移组 ;临床Ⅲ期的病人P5 3蛋白阳性率明显高于临床Ⅰ和Ⅱ期的病人。并且有P5 3蛋白表达的病人其生存期明显短于无P5 3蛋白表达的病人。p2 1WAF1亦与病人的生存期有关 ,存在p2 1WAF1表达的病人其生存期明显长于无p2 1WAF1表达的病人。结论 胆管癌中P5 3,P2 1WAF1蛋白表达与预后密切相关 ,提示二者可能在胆管癌的发展过程中起到重要作用。p5 3,p2 1WAF1之间未见明显相关性 ,说明可能存在非p5 3依赖的p2
Objective To clarify the clinical significance of p21 WAF1 and the relationship between it and p53. Methods Samples of human cholangiocarcinoma (HC) tissue and paired normal bile duct tissue adjacent to the tumor from 30 patients with HC were employed in this study. In situ hybridization (ISH) was used to detect the p53 and p21 mRNA expression. Immunohistochemistry (IHC) was applied to analyze p53 gene mutation and P21 protein expression. Results p21 WAF1 And p53 gene protein expression was detected in 36.7% (11/30) and 53.3% (16/30) of the carcinoma specimens, respectively, and none of the paired tissue by IHC. p53 Positivity was related to local lymph node metastasis. Comparing with non-lymph node metastatic group, p53 positivity in metastatic group was significantly higher. p53 Positivity of cholangiocarcinoma in clinical stage III was significantly higher than that in clinical stages I and II. The survival time was significantly shorter in patients with P53 protein expression than in those without. It was found that p53 expression was not associated with p21 WAF1 expression. Conclusions P53 Positivity may be correlated with tumor development but not tumor occurrence. The fact that p53 expression was not associated with p21 WAF1 expression indicates that p53-independent activation of p21 WAF1 may exist.
出处
《中华肝胆外科杂志》
CAS
CSCD
2000年第6期442-446,共5页
Chinese Journal of Hepatobiliary Surgery
基金
国家杰出青年基金资助