摘要
目的:探讨β3肾上腺素能受体(β3-adrenoreceptor,β3-AR)激动对慢性心力衰竭(心衰)大鼠心脏重构及血流动力学的影响。方法:24只Wistar大鼠分为假手术组(n=6),其余大鼠用主动脉缩窄法建立慢性心衰模型后,分为心衰组(n=8)和β3-AR药物组[n=8,经尾静脉注射β3-AR特异性激动剂BRL-37344 0.4 nmol/(kg·min),注射10 min,每周2次,连续4周],随后用超声心动图检测心脏结构和功能,应用BL-410生物机能实验系统完成血流动力学检测。结果:①3组大鼠超声心动图参数的变化:与假手术组或心衰组比较,β3-AR药物组的左心室舒张末期内径、左心室收缩末期内径、室间隔舒张末期厚度、左心室后壁舒张末期厚度和左心室重量均显著增加(P<0.05或P<0.01),而左心室射血分数和短轴缩短率均显著降低(P<0.05),差异均有统计学意义。②3组大鼠血流动力学参数的变化:与假手术组或心衰组比较,β3-AR药物组的左心室舒张末压、左心室内压最大下降速率、平均肺动脉压均显著升高(P<0.01),而左心室收缩末压、左心室内压最大上升速率均显著降低,差异均有统计学意义(P<0.01)。结论:β3-AR特异性激动剂BRL-37344加重心衰大鼠的心脏重构及促进衰竭心脏收缩功能下降,促进心衰大鼠血流动力学恶化。
Objective：To investigate the effect of selective β3-adrenoreceptor （β3-AR） agonist BRL-37344 on cardiac remodeling and hemodynamic in chronic heart failure （CHF） model in rats.Methods：A total of 24 male Wistar rats were divided into 3 groups.Sham operation group,n=6,the rest 18 rats were established for CHS model and 16 survival rats were further divided in 2 groups,CHF group and CHF with BRL-37344 group,in which the rats received BRL-37344 at 0.4 nmol/kg· min,twice/week for 4 weeks.n=8 in each group.Echocardiography was performed to assess the cardiac structure and function,some hemodynamic indicators were measured by BL-410 biological experimental system in all animals.Results：① By echocardiography examination,compared with Sham operation group or CHF group,CHF with BRL-37344 group showed increased LVEDD,LVESD,left ventricular thickness at diastole and left ventricular weight,P＜0.05 or P＜0.01; while decreased LVEF,P＜0.05.③ For hemodynamic changes,compared with Sham operation group or CHF group,CHF with BRL-37344 group had increased LVEDP and MPAP,P＜0.01; while decreased LVESP,P＜0.01.Conclusion：β3-adrenoreceptor agonist BRL-37344 may aggravate cardiac remodeling and systolic function and further worsening the hemodynamic in CHF model in rats.
出处
《中国循环杂志》
CSCD
北大核心
2013年第6期455-457,共3页
Chinese Circulation Journal
