牙龈卟啉单胞菌—脂多糖对人脐静脉内皮细胞增殖和凋亡的影响

Effect of Porphyromonasgingivalis Lipopolysaccharide on Proliferation and Apoptosis in Human Umbilical Vein Endothelial Cells in vitro

目的:观察牙龈卟啉单胞菌-脂多糖(P.g-LPS)对人脐静脉内皮细胞(HUVEC)增殖和凋亡的影响。方法:在体外将P.g-LPS与HUVEC共培养,根据P.g-LPS浓度分为空白对照组、50 pg/ml组、100 pg/ml组、1ng/ml组及10 ng/ml组。培养36 h后采用甲基噻唑基四唑(MTT)法及流式细胞仪分别测定细胞增殖活力及凋亡情况,并通过免疫印迹标记法(Western blot)检测caspase-3激活情况。结果:经P.g-LPS处理后,HUVEC增殖活力较空白对照组明显下降(P<0.01),且随着P.g-LPS浓度增加,HUVEC增殖活力也逐渐下降(P<0.01),而细胞发生凋亡及caspase-3激活情况却随之增加。结论:P.g-LPS可抑制内皮细胞增殖活力,caspase-3激活从而诱导内皮细胞产生凋亡。

Objective： To study the effect of porphyromonasgingivalis lipopolysaccharide （P.g-LPS） on proliferation and apoptosis in human umbilical vein endothelial cells （HUVEC） in vitro. Methods： The HUVEC were cultured with different concentrations of P.g-LPS as P.g-LPS 0 pg/ml （Control group） and P.g-LPS 50pg/ml, 100pg/ml, lng/ml and lOng/ml groups respectively. After 36 hours incubation, the cell proliferation viability and apoptosis were detected by MTT method and flow cytometry assay, the caspase-3 activation was examined by Western blot analysis. Results： Compared with Control group, the P.g-LPS treated HUVEC showed obviously decreased proliferation viability, P〈0.01. While with the increased P.g-LPS concentrations, the cell proliferation viability decreased accordingly, P〈0.01, and the cell apoptosis and caspase-3 activation were increased accordingly. Conclusion： P.g-LPS may inhibit endothelial cell proliferation viability, promote caspase-3 activation and therefore, inducing cell apoptosis in vitro.