大鼠Akt1基因合成及载体构建的实验研究

Experimental Study on the Gene Synthesis and Eukaryotic Expression Vector Construction of Rattus Akt1

根据Genebank提供的大鼠Akt1基因序列,用PCR方法对大鼠Akt1序列进行基因合成,再将其克隆到pGCMV/MCS/RFP/Neo质粒中,而后进行NheI和BamHI双酶切法验证,为今后Akt1基因在生命科学实验中的研究打下基础。

The Aktl gene was synthesized according to the gene sequences of rats from Genebank, followed by subcloning into pGCMV/MCS/ RFP/Neo expression vector. It was identified by using NheI ＋ BamHl double digestion and by sequencing. The research lays a foundation for manufacturing the protein and studying its function.