α-黑素细胞刺激素体外对星形胶质细胞产生NO和前炎性细胞因子的影响

Effects of α-melanocyte stimulating hormone on the production of NO and proinflammatory cytokines in astrocytes in vitro

目的 :研究α 黑素细胞刺激素 (α MSH)对LPS诱导星形胶质细胞产生NO和前炎性细胞因子的影响 ,探讨α MSH的抗炎作用机制。方法 :分别用LPS或α MSH +LPS处理体外培养的大鼠脑星形胶质细胞 ,用Griess试剂测定NO ,以MTT显色法检测IL 1、IL 6和TNF α ,采用半定量RT PCR检测MIFmRNA表达。结果 :体外培养的星形胶质细胞在LPS刺激下产生NO、IL 1、IL 6、TNF α和表达MIFmRNA显著增高 ;若同时给予LPS和α MSH ,可明显降低NO、IL 1、IL 6和TNF α的产生以及MIFmRNA表达。结论 :提示α

Objective:In order to explore the anti inflammatory mechanisms of α melanocyte stimulating hormone (α MSH), the effects of α MSH on the production of NO and proinflammatory cytokines in astrocytes induced by LPS were investigated Methods:Rat brain astrocytes cultured in vitro were stimulated with LPS or given α MSH with LPS stimulation NO produced in astrocytes was tested with Griess reagent IL 1, IL 6 and TNF α secreted from astrocytes were examined by MTT assay The expression of macrophage migration inhibitory factor (MIF) mRNA was examined with semiquantitative RT PCR analysis Results:The production of NO, IL 1, IL 6, TNF α and the expression of MIF mRNA were significantly increased in astrocytes stimulated with LPS If giving α MSH with LPS stimulation, the production of NO, IL 1, IL 6, TNF α and the expression of MIF mRNA were markedly decreased Conclusion:[WT5”,6BZ]It is suggested that the inhibitory actions of α MSH on the production of NO and proinflammatory cytokines in astrocytes are related to the inhibitory effects of α MSH on inflammation in central nervous system