机械振荡刺激对菊花愈伤组织营养元素吸收的研究

STUDIES OF MECHANICAL VIBRATION STIMULATION INFLUENCEING THE GROWTH ON CHRYSATHEMUM CALLUS

菊花茎尖来源的愈伤组织经机械振荡刺激后其生长速率显著高于对照组，但存在一个最佳振荡频率。在这个最佳振荡频率下，愈伤组织对营养元素的吸收率最高，而后随着振荡频率的增大，其对营养元素的吸收率又降低，表现出抑制效应，这进一步说明了机械振荡刺激对愈伤组织的生长具有双向效应。为了进一步探讨机械振荡的生物学效应，对细胞内的钙离子的分布进行了化学定位，在细胞质和液泡内的钙离子的分布存在很大的差异，并初步探讨了机械振荡刺激加速对营养元素吸收的可能机理。

Stem tip callus of chrysanthemum treated with mechanical vibration grow more rapidly than non-treatment callus in vitro. Mechanical vibration treatment enhances the nutriment such as P, K, Ca, Mg element absorption rate of callus in culture. In our experiments we found an optimum frequency, the absorption rate is the highest in this frequency. However, with the frequency increasing, the absorption rate begins to decrease. It is shown that the mechanical vibration has the bidirectional effect. In order to explore the mechanism of the biological eftects caused by mechanical vibration, We studied the distribution of Ca2+ sediments with in cells by electron microscopy and found the Ca2+ distribution was notably different between the control group and treatment groups. In this paper, we also preliminarily approach the mechanism of callus proliferation by mechanical vibration.