无溶剂体系酶促酯交换合成富含1,3-二山嵛酸-2-油酸甘油酯的工艺研究

Synthesis of 1,3- dibehenoyl- 2-oleoyl glycerol via enzymatic interesterification in solvent-free system

研究了无溶剂体系中固定化1,3-特异性脂肪酶催化山嵛酸甲酯与高油酸葵花籽油合成富含1,3-二山嵛酸-2-油酸甘油酯(BOB)的工艺,考察了酶添加量、底物摩尔比、反应时间、反应温度、酶的初始水分活度对BOB和甘油二酯含量影响。以甘油二酯含量较低而BOB含量较高为优,确定理想的工艺条件为:Lipozyme RM IM添加量10%(以底物质量计),底物(山嵛酸甲酯与高油酸葵花籽油)摩尔比2∶1,反应时间8 h,反应温度65℃,酶的初始水分活度由LiCl饱和盐溶液控制。在理想的工艺条件下,反应产物中BOB含量为25.25%,甘油二酯含量为2.72%。

Products rich in 1, 3 -dibehenoyl -2 -oleoyl glycerol （BOB） were produced via enzymatic interesterification of high oleic acid sunflower seed oil and behenic acid methyl ester catalyzed by 1, 3 - regiospecific lipase in solvent -free system. The effects of enzyme dosage, molar ratio of behenic acid methyl ester to high oleic acid sunflower seed oil, reaction time, reaction temperature and initial water activity of the enzyme on contents of BOB and diaeylglycerol were investigated. The product with high content of BOB and low content of diacylglycerol was expected. The results showed that the optimal con- ditions were determined as follows： Lipozyme RM IM dosage 10% （based on the mass of substrates）, molar ratio of behenic acid methyl ester to high oleic acid sunflower seed oil 2： 1, reaction time 8 h, reac- tion temperature 65 ~C, and initial water activity of the enzyme was controlled by saturated LiC1 solution. Under the optimal conditions, the contents of BOB and diacylglycerol in the reaction product were 25.25% and 2.72%, respectively.