全反式维甲酸立体稳定脂质体的制备

PREPARATION OF STERICALLY STABILIZED LIPOSOMES CONTAINING ALL-TRANS RETINOIC ACID

目的 制备全反式维甲酸 (RA)的立体稳定脂质体 ,初步研究其物理化学性质。方法 用DSC、1HNMR、激光粒径测定仪研究其微观结构、粒径大小及其分布 ,并用TEM拍摄。结果 制备的普通和立体稳定脂质体粒径分别约为 0 35和 0 42 μm ,电镜下可观察到脂质体的立体稳定结构。 结论 立体稳定脂质体中磷脂极性头部被吐温 80的聚氧乙烯基所覆盖 ,吐温 80掺入脂质膜 ,伸出的亲水性聚氧乙烯基提供了RA立体稳定脂质体的立体位阻。

AIM To prepare sterically stabilized liposomes containing all trans retinoic acid, and to study its physicochemical properties. METHODS The microstructure, the particle size and its distribution was determined by DSC, 1HNMR and 90 Plus Particle Size Analyzer and a TEM photo was taken. RESULTS The size of conventional liposomes and sterically stabilized liposomes were about 0 35 and 0 42 μm. The sterically stabilized structure can be confirmed by TEM photo. CONCLUSION The stericallly stabilized liposomes containing all trans retinoic acid can be prepared by combination of film casting and freeze drying.