西他沙星代谢物的LC-MS法研究

Simultaneous identification and separation of sitafloxacin metabolites by LC-MS

采用 L C- MS技术分离和鉴定新氟喹诺酮类抗菌剂西他沙星体内代谢物。试验对口服西他沙星后大鼠、狗和人的尿样及胆汁分别进行分离和测定。结果在线分离和鉴定了西他沙星的 9个主要代谢物 (M1～M9) ,为西他沙星体内代谢研究及其它新氟喹诺酮类化合物的研制提供了良好的筛选结构群。

Sitafloxacin metabolites were simultaneously separated and identified by LC MS. After being administrated with sitafloxacin, the urine and bile samples of rat, dog, monkey and human were determined respectively. Nine metabolites (M1~M9) of sitafloxacin have been identified. It is not only valuable for studying the metabolism of sitafloxacin but also provides structural candidate for developing new fluoroquinolones.