联合检测乙型病毒性肝炎前S1抗原、大蛋白抗原的临床意义

Clinical significance of detection HBV Pre-S1 antigen and HBV-LP for the patients with hepatitis B virus infection

目的:研究联合检测乙肝前S1抗原(Pre-S1Ag)、大蛋白抗原(HBV-LP)的临床意义。方法:对274例乙肝患者采用PCR法检测HBV-DNA的含量,ELISA法检测Pre-S1Ag、HBV-LP、HBsAg,并采用速率法检测血清丙氨酸转氨酶(ALT)的水平。结果:274例乙肝患者Pre-S1Ag、HBV-LP及HBV-DNA阳性率相比较差异无统计学意义(P>0.05)。Pre-S1Ag与HBV-LP双阳性中HBV-DNA阳性的比例显著高于Pre-S1Ag阳性和HBV-LP阳性(P<0.05)。Pre-S1Ag与HBV-LP双阳性患者中ALT异常的比例显著高于Pre-S1Ag阳性和HBV-LP阳性(P<0.05)。结论:联合检测乙肝患者Pre-S1Ag和HBV-LP对判断HBV复制及患者的肝功能具有重要的临床意义。

Objective：To study the clinical significant of detection HBV Pre-S1 and HBV-LP for the patients of hepatitis B. Methods： For the 274 patients with hepatitis B virus infection,the HBV Pre-S1Ag and HBV-LP was detected with ELISA,and the levels of alanine aminotransferase（ALT）were measured by an automated biochemistry analyzer. Results： No significant difference was found among the detectable positive rates of HBVDNA,Pre-SlAg and HBV-LP in patients with hepatitis B virus infection. In the both Pre-S1Ag and HBV-LP positive group,the detectable rates of HBV-DNA were significantly higher than the Pre-S1Ag positive group and the HBV-LP positive group. The detectable rates of patients with high levels of ALT in the both Pre-S1Ag and HBV-LP positive group were significantly higher than the Pre- S1Ag positive group and the HBV- LP positive group.Conclusion： Detection of Pre-S1Ag and HBV-LP together have important clinical significances in diagnosing infection of HBV and analyzing situation of reproduction of HBV and observing the liver function.