PLGA微球复合明胶组织工程支架缓释蛋白药物的研究

Encapsulation of Proteins in PLGA Microsphere-gelatin Composite Scaffold

目的:研究PLGA微球复合明胶支架对蛋白药物的释放影响。方法:将模型蛋白BSA通过复乳法制备成缓释PLGA微球,然后将微球埋置于明胶支架中,形成担载蛋白的PLGA微球复合明胶组织工程支架。考察复合支架体外蛋白释放行为,并用Micro BCA法定量测定释放的BSA量,采用β-半乳糖苷酶催化ONPG的方法检测制备前后蛋白的活性,并与不含PLGA微球直接担载蛋白的支架做对照。结果:PLGA微球复合支架蛋白的包封率能达到73.2%,其中第一天释放20%,对蛋白活性的保持达到70%以上。结论:微球复合明胶支架可以改善一般组织工程支架蛋白药物的突释,提高蛋白药物在制剂,贮存,释放过程中的稳定性。

Objective： To investigate the effect of PLGAmicrospheres composite scaffold on the releasing behavior of protein drug. Methods： BSA was encapsulated into PLGA microspheres by W/O/W emulsion methods, then embedding the microspheres into gelatin to form 3-D composite scaffolds. The release behavior of the composite scaffold in vitro was detected by MicroBCA method and the bioactivity of β-galactosidase （β-gal） was determined by ONPG method. The results were compared with the composite scaffolds without microspheres. Results： Drug loading for the experiment group reached 73.2%, with first day release amount is 20%, the bioactivity of β-gal preserved more than 70 %. Conclusion： Microspheres composite scaffold can improve release behavior of protein drug in tissue engineering, increase drug loading and drug stability during preparation, storation and release process.