乌鲁木齐地区乳腺癌与人乳头瘤病毒亚型感染的相关性研究

In Urumqi Region,the Correlation of Infection with Human Papilloma Virus Subtype of Breast Cancer Research

目的:对本地区乳腺癌患者癌组织进行人乳头瘤病毒(HPV)检测,对HPV亚型与乳腺癌的相关性进行研究。方法:选择本地区2010年1月-2013年1月182例乳腺癌患者作为研究组,同时选择30例乳腺良性肿瘤患者作为对照组,均分型基因芯片检测系统对提取的DNA进行分型检测,观察两组患者HPV感染情况及感染亚型。结果:两组患者HPV阳性病例67例,其中研究组阳性66例,阳性率为36.26%,对照组阳性1例,阳性率为3.33%;研究组阳性率明显高于对照组,两组比较差异具有显著性(P<0.05)。亚型检测结果显示,共检出7种HPV亚型,其中高危型5种,分别为HPV16、HPV18、HPV 58、HPV 51和HPV 56型,低危型两种,分别为HPV6、11两种。研究组高危型61例,低危型5例,对照组1例为HPV6。I期、Ⅱ期、Ⅲ期、Ⅳ期乳腺癌患者HPV阳性率分别为16.28%、19.71%、62%、100%,Ⅲ期、Ⅳ期患者的阳性率明显高于I期、Ⅱ期,不同分期阳性率比较差异具有显著性(P<0.05)。结论:乳腺癌患者癌组织中HPV阳性率明显偏高,且绝大多数为高危亚型,而且HPV阳性率与病理分期呈正相关,说明本地区HPV感染与乳腺癌的发生与发展具有一定的相关性,两者的相关性还有待进一步探索。

Objective： To detect breast cancer tissues in the region of human papillomavirus （HPV）, and to study the relation of HPV subtypes with breast cancer. Methods： In the region in January 2010, January 2013, 182 patients with breast cancer were selected as the study group, 30 cases of benign breast tumor patients were selected as a control group, extracted DNA genotyping were studied by the sharing gene chips, HPV infection and infection subtypes in patients were observed. Results： Patients with in the two groups were 67, 66 cases in the study group was positive, the positive rate was 36.26%, one case is positive in control group and the positive rate was 3.33%; that in study group was significantly higher than that in the control group, the difference is significant （P 〈0.05）. Subtype test results showed seven types of HPV subtypes with were detected, including high-risk types ：HPV16, HPV18, HPV 58, HPV 51 and HPV 56 type; two low-risk types： HPV6, HPV 11. High-risk types in study group were 61 cases, and low-risk types were 5 cases. In the control group, HPV6 was 1 case. The rates of Phase I, Phase II, Phase III, Phase IV breast cancer patients with HPV-positive were 16.28%, 19.71%, 62%, 100%, respectively. The rates of Phase III, IV patients with positive were significantly higher than those of Phase I, Phase II, the installments positive rate and the difference was significant （P 〈0.05）. Conclusion： The rate of HPV-positive in cancer tissue of breast cancer patients is significantly higher, and the vast majority of high-risk subtypes of HPV prevalence was positively correlated with pathology, indicating that the region between HPV infection and the occurrence and development of breast cancer is related with each other, the correlation between them remains to be explored further.