开口箭对小鼠5-羟色胺分泌的影响

Effects of Tupistra chinensis on 5-hydroxytryptamine secretion in mice

目的：采用灌胃、石蜡切片、免疫组化染色和ELISA等方法，探讨开口箭对小鼠5-羟色胺（5-HT）分泌的影响．方法选取20～22g健康昆明小鼠20只，均分成A，B，c，D4组，分别以蒸馏水和0．25、0．50、1．00mg／mL的开口箭灌胃，0．20mL／次／d，连续灌胃7d．第8d对全部小鼠摘眼球取血，ELISA检测血清5-HT质量浓度；取血后立即处死小鼠，取十二指肠，4％甲醛固定，石蜡切片，免疫组化染色，镜下统计5-HT阳性细胞数量．结果与A组相比，B，C，D组小鼠血液5-HT质量浓度逐渐降低，十二指肠肠壁组织中5-HT阳性细胞数也明显减少．结论开LI箭会下调小鼠5-HT的分泌．

Objective： By the methods of gavage, paraffin section, immunohistochemical staining, and ELISA, the effects of Tupistra chinensis on 5-hydroxytryptamine secretion was studied in mice. Methods： Twenty healthy Kunming mice weighing 20-22 g were selected and divided into A, B, C and D groups on average. In a serial 7 days, drenched respectively distilled wa ter to A group mice and 0.25, 0.50, 1.00 mg/ml Tupistra chinensis diluted solution to B, C and D group mice, with the dose of 0.2 ml/t/d. At 8th day, the blood of all the mice was collected by the method of eyeball picking and the content of 5- hydroxytryptamine in blood serum was analysis by the method of ELISA, and meanwhile, all of mice were killed rapidly, the duodenums were harvested, fixed in 4 ~//00 formaldehyde, made paraffin sections, stained by immunohistochemical methods, and 5-hydroxytryptamine positive cells were observed and count under light microscope. Results： From A to D group mice, the content of 5-hydroxytryptamine in blood serum were decreased gradually, and also the quantities of 5-hydroxytryptamine posi- tive cells in duodenums were less and less. Conclusion： Tupistra chinensis can downregulate secretion of 5-hydroxytryptamine in mice.