摘要
目的:探讨DNA-PK和CHK1对卵巢癌Skov3的细胞周期阻滞的机制。方法:通过对DNA-PK shRNA和CHK1 shRNA的构建及有效干扰载体的筛选,流式细胞仪检测G2期。结果:转染DNA-PK shRNA的Skov3细胞和Hela细胞经2Gy X射线照射后进入G2期的细胞比率无差异;转染CHK1 shRNA的Skov3细胞经2Gy X射线照射后4h进入G2期的细胞比率显著高于Hela细胞(t=2.618,P=0.026),28h时明显低于Hela细胞(t=2.705,P=0.022);转染DNA-PK shRNA和CHK1 shRNA的Skov3细胞经2Gy X射线照射后4h进入G2期的细胞比率显著高于Hela细胞(t=2.965,P=0.014),28h时明显低于Hela细胞(t=2.302,P=0.044)。结论:干扰DNA-PK和CHK1的表达可增强卵巢癌细胞对辐射的敏感性。
Objectives: To explore the mechanism of DNA - PK and CHK1 on cell cycle block in ovarian cancer Skov3 cells. Method: We created DNA - PK shRNA and CHK1 shRNA and filtered the effective interference vectors. We detected the G2 phase by flow cytometry. Results: The proportions of Skov3 cells and Hela ceils transfected with DNA - PK shRNA and radia- ted by 2Gy X that entered the G2 phase showed no significant difference. The proportion of Skov3 cells transfected with CHK1 shRNA and radiated by 2Gy X that entered the G2 phase was significantly higher than of that of Hela cells at 4h of post - radiation ( t = 2.618, p = 0. 026), and significantly lower than of that of Hela cells at 28h of post - radiation ( t = 2. 705, P = 0. 022). The proportion of Skov3 cells transfected with DNA - PK shRNA and CHK1 shRNA that entered the G2 phase was significantly higher than that of Hela cells ( t = 2. 965, P = 0. 014) at 4h of post - radiation, and was significantly lower than that of Hela cells (t =2. 302, P =0. 044) at 4h of post - radiation. Conclusion: The interference with DNA - PK and CHK1 expression can en hance the sensitivity of ovarian cancer cells to radiation.
出处
《中国性科学》
2013年第11期26-29,共4页
Chinese Journal of Human Sexuality
基金
黑龙江省卫生厅项目(2012-489)