摘要
创造新的种质资源材料,对于加速小麦遗传改良研究具有重要意义。利用60Coγ射线对小麦幼胚愈伤组织进行5-30 Gy剂量的辐照处理,并对其生物学效应进行研究。结果表明,辐照处理对小麦幼胚愈伤组织的分化产生很大影响,随辐照剂量增大,愈伤组织的分化率逐渐下降。对M2代分离群体进行观察,获得叶片、茎秆、株型和穗型等形态性状发生变异的突变体,以及生育期等生理性状发生改变的变异株。这些突变体材料为小麦新品种的选育及其功能基因组学研究提供了很好的基础材料。
Background： Creation of new germplasm derived from wheat can accelerate the genetic breeding of wheat. Purpose： Samples of the callus from immature embryo were irradiated by 60Coγ-ray to study their biological effects. Methods： The calluses were irradiated at the doses of 5 Gy, l0 Gy, 15 Gy, 20 Gy and 30 Gy （dose rate 10 Gy/min）. And the physiological characters in ME generation were observed. Results： The results showed that the rates of callus differentiation decreased with the increasing of doses. Morphological variations including leaf, stalk, plant type, panicle type and various physiological characters were observed in the ME generation. Conclusion： The mutants were helpful for gene identification and gene functional analysis, and for breeding of new wheat variety as well.
出处
《核技术》
CAS
CSCD
北大核心
2013年第12期19-24,共6页
Nuclear Techniques
基金
作物生物学国家重点实验室开放基金(2012KF09)
中国科学院技术生物所联合青年基金(Y29LH24892)资助
