20种蚕豆样品蛋白质含量与其蛋白组分的分子质量

Protein Content and Relative Molecular Mass of their Subunit from Twenty Broad Bean Varieties Grown in China

采用微量凯氏定氮、蛋白质分级提取、变性凝胶电泳（SDS—PAGE）等技术研究了我国蚕豆主产区20种代表性蚕豆样品的蛋白质含量，蚕豆蛋白质中水溶性、盐溶性以及醇溶蛋白质的质量分数，以及蚕豆水溶性与盐溶性蛋白质亚基的分子质量分布。结果表明：中国蚕豆主产区20种蚕豆样品的蛋白含量范围26．48-32．51g／100g，20种蚕豆蛋白含量差异显著（P〈0．05）。20种蚕豆样品中的水溶性蛋白、盐溶性蛋白、醇溶蛋白的含量分别为88．5％～93．5％、4．3％～8．6％、0．3％～2．5％、其他非蛋白氮为0．7％-3．6％。不同品种间的蚕豆水溶性、盐溶性蛋白亚基组成具有一定的差异；SDS—PAGE电泳分析，蚕豆水溶性蛋白共分离出10～19条迁移率不同的条带，亚基分子质量集中在43．0～95．0ku之间，盐溶性蛋白共分离出7～16条不同迁移率的条带，因品种不同而不同。分子质量为65、55、48、43、32ku的谱带在20种蚕豆盐溶蛋白中均被检测出来，为共有带。

Here adopt micro Kjeldahl nitrogen, protein extraction procedure, denaturing gel electrophoresis （ SDS - PAGE） and other techniques to study the protein content of 20 typical varieties of broad beans which are produced in main broad bean producing areas of China, the content of faba bean protein water - soluble, salt - soluble and alchol - souluble protein, as well as the distribution of molecular qualities of the broad bean water - soluble salt soluble protein subunits. The results showed that the protein content of the 20 faba bean varieties ranged from 26.48 to 32.51 g/100 g. In addition, 20 faba protein content was significantly different （P 〈 0.05 ）. Water- soluble pro- teins, salt- soluble proteins, alchol- souluble protein content in broad bean protein were 88.5%-93.5% ,4.3%- 8.6%, 0.3%-2.5% respectively. The content of other non - protein nitrogen was 0.7%-3.6%. There were some certain differences between the subunit composition of different varieties of broad bean water - soluble, salt - soluble protein , they showed some polymorphism; SDS- PAGE electrophoresis demonstrated that there were 10 - 19 bands with different mobilities, the subunit molecular of broad beans water - soluble protein were concentrated between 43. 0 to 95.0 ku, salt - soluble protein isolated 7 - 16 strips with different migration rates, varied with varieties. Relative molecular qualities of 65, 55, 48,43,32 ku bands were all detected in the 20 faba salt - soluble protein, they were the common bands.