银杏叶提取精制工艺研究

Study on ProductionTechnology of Ginkgo Biloba Extract and Refining

目的：建立银杏叶提取物的精制工艺。方法：以总黄酮醇苷和萜类内酯量为考察指标，采用L。（3-4）正交试验，探索乙醇提取最佳工艺。结果：采用5倍量90％（v／v）浓度的乙醇回流2h，提取4次；提取液浓缩至相对密度1．10（65～75℃）后，静置8h，去除相应杂质（叶绿素、树脂）；使用DA201大孔吸附树脂，750．4（v／v）乙醇作为洗脱剂对粗提物进行纯化；再用0．2％活性炭80℃保温30min，除去银杏酸。结论i该工艺稳定、可行，适宜进行工业化生产。

Objective： The extraction technology of extract of Ginkgo biloba leaves was established. Methods： The extraction process was optimized through L9 （34） orthographic experimented design by selecting the content of flavonoids and terpenoids as the analysis indexes. Results：The optimal process conditions were as follows： Recirculation, added 5 times of Ginkgo biloba L with 90% ethanol for 2 h and four times. The ethanol solution of ginkgo leaf extracts was inspissationed to 1.10（65-75℃ ）, stood, and the impurities such as chlorophyll, and resins etc. , were then removed. The preliminary ethanol concentrates were further purified through a macroporous resin chromatograph, which adsorbed the ginkgo leaves extracts, with an elution by 75 % ethanol. Finally, incubate with 0.2 % of activated carbon 80℃ for 30 rain, removed ginkgolic acid. Conclusion：The extraction process was stable, feasible and suitable for manufacturing scales.