正交试验设计优化沙棘总黄酮提取工艺

Orthogonal Design for the Extraction Process of Total Flavonoids from Seabuckthorn

目的:通过比较超声、索氏及回流提取三种方法的黄酮得率,确定最佳沙棘黄酮提取方法,再确定该法萃取沙棘黄酮的最优工艺条件。方法:通过单因素和L9(34)正交实验设计进行提取工艺优化。结果:超声法优于索氏和回流提取法,超声法最优工艺为:提取功率60W,甲醇提取2次,每次1h,料液比1∶8。结论:超声提取法具有黄酮产率高、省时、简便、溶剂用量少等优点。

Objective：To compare ultrasound, Soxhlet and reflux extraction yield of flavonoids of three methods to determine the best extraction method and optimum of seabuckthorn flavonoids. Methods： Through single factor and orthogonal experimental method to do comparative study. Results： Ultrasonic method is superior to Soxhlet and reflux extraction, ultrasonic optimal process： extraction power 60w, methanol extract two times, each time l h, solid-liquid ratio 1：8. Conclusion：The ultrasonic ex- traction has flavonoids high yield, saving, simple, less solvent, etc.