抗阻训练对衰老大鼠腓肠肌Bcl-2BaxmRNA及线粒体膜电位的影响

Effects of Resistance Training on the Aging Rats Gastrocnemius of Apoptosis Regulatory Genes and Mitochondrial Membrane Potential

目的:探讨抗阻训练对衰老大鼠腓肠肌Bcl-2、Bax mRNA表达及线粒体膜电位(ΔΨmt)的影响。方法:以雄性SD大鼠为研究对象,饲养至18月龄后通过8周抗阻训练干预,分别以0%、30%、50%、70%最大负重进行间歇跑台运动,坡度为35°,跑速15 m/min,隔天一次。8周末检测腓肠肌Bcl-2、Bax mRNA表达水平,JC-1脂质荧光染料法检测线粒体膜电位。结果:与衰老安静组相比,训练组衰老大鼠腓肠肌Bcl-2/Bax mRNA比值升高,线粒体膜电位(ΔΨmt)下降的细胞百分率减少。其中,30%、50%最大负重抗阻训练组的Bcl-2/Bax mRNA比值升高,且具有统计学意义(P<0.01,P<0.05);0%、30%、50%最大负重抗阻训练组衰老大鼠线粒体膜电位下降的细胞百分率的差异有统计学意义(P<0.01)。结论:低、中等负重抗阻训练能上调衰老过程中腓肠肌Bcl-2/Bax mRNA比值,减少线粒体膜电位(ΔΨmt)下降的细胞百分率,对腓肠肌细胞凋亡的发生有明显的调控作用。

Objective ： To study the effects of resistance training on the aging rats gastrocnemius of apoptosis gene Bcl-2, Bax mRNA expression and mitochondrial membrane potential （△ψmt）. Method： The male SD rats as the research object, after feed to 18 months, they were assigned as 0%, 30%, 50%, 70% maximum weight resistance training intervention. The gastrocnemius apoptosis gene Bcl-2, Bax mRNA expression and mitochondrial membrane potential （AWmt） were tested after a period of quote of the day for 8 weeks treadmill training with the speed of 15 m/min, slope as 35°. Results： Compared with the aging control group, Bcl-2/Bax ratio of apoptosis regulatory genes and mitochondrial membrane potential increased in the resistance training groups, among them with 30%, 50% maximum weight resistance training groups Bcl-2/Bax ratio increased extremely significance or significantly （P〈0.01, P〈0.05）, and their △ψmt increased extremely significance in 0%, 30%, 50% maximum weight resistance training groups （P〈0.01）. Conclusion： Low and moderate intensity resistance training may be improved apoptosis regulatory genes Bcl-2/Bax ratio and mitochondrial membrane potential, to regulate the occurrence of gastrocnemius apoptosis significantly.