棕帘固定化环状芽孢杆菌ATCC21783制备CGTase

Cyclodextrin glucanotransferase production of Bacillus circulans ATCC 21783 immobilized on brown shade

棕帘具有环境友好、耐腐蚀性强、可循环利用等特点,常用于水产养殖附苗。文中首次采用棕帘对细胞进行固定化,研究固定化过程中的接种量、固定化时间以及棕帘的投放数量对环糊精葡萄糖基转移酶的影响。结果发现,在50 mL培养基中,接种量为3%、固定化时间为1 d、棕帘(长2.5 cm,宽2 cm,高5 mm)的投放数量为2时,固定化细胞产酶量较高为5 519.05 U/mL。与现有报道的丝瓜瓤、丝瓜瓤-海藻酸钠、棕帘-海藻酸钠等固定化载体相比,棕帘具有成本低、操作简单、具有较好的操作稳定性等特点,在第7个循环中酶活性仍可达到第1个循环时的83.5%,有利于工业化推广应用。

There are lots of fine features on brown shade,such as friendly environment,corrosion resistance,recyclable usage,etc.. It is commonly used as matrices of seed-adhering in aquaculture. This research is the first time to immobilize cells on brown shade. The effect on cyclodextrin glycosyltransferase of the amount of inoculum,immobilization time,and the number of the brown shade was studied in this article. It was found that the immobilized bacterial cells could get higher levels of enzyme production as 5519. 05U / mL under the condition of inoculation of 3%,immobilization time of 1 day,the number of brown shade of 2 pieces. Compared to existed reports on matrices of loofa sponge,loofa sponge-sodium alginate,brown shade-sodium alginate,brown shade has a characteristic with low cost,simple operation,and good operational stability,etc. In the first seven cycles,the activity of the last cycle could be attained as 83. 5% of the first one,thus making the process potentially suitable for industrial scale-up.