淀粉脱支后环化制备大环糊精

Cyclization reaction by starch debranching enzyme in cycloamylose production

以普通玉米淀粉、木薯淀粉、高直链玉米淀粉为底物制备大环糊精,先利用异淀粉酶将这3种底物脱支处理以后,再利用栖热水生菌4-α-糖基转移酶的环化作用来生产大环糊精。结果表明:经过脱支处理以后,普通玉米淀粉大环糊精的最高转化率从20.3%增加到33.6%(1.7倍);木薯淀粉大环糊精的最高转化率从16.82%增加到32.4%(1.9倍);高直链玉米淀粉大环糊精的最高转化率从24.53%增加到44.07%(1.8倍)。因此,该方法是种有效的大环糊精生产方法,具有工业化应用的潜能。

Corn starch,cassava starch and amylomaize were utilized to produce cycloamylose in this study. These three substrates were debranched by isoamylase first. Then they were treated with 4-α-glucanotransferase to produce cycloamylose. The results indicated that after debranching,the maximum conversion yield of cycloamylose from corn starch increased from 20. 3% to 33. 6%（ 1. 7 folds）; the maximum conversion yield of cycloamylose from cassava starch increased from 16. 82% to 32. 4%（ 1. 9 folds）; the maximum conversion yield of cycloamylose from amylomaize increased from 24. 53% to 44. 07%（ 1. 8 folds）. Therefore,it was an efficient method to produce cycloamylose by combined treatment of isoamylase and 4-α-glucanotransferase. The study also showed the method has a great potential in the industrial scale.