复方苦荞螺旋藻片的降糖作用及安全性

Research on the decreasing blood glucose effect and safety evaluation of Buckwheat-Spirulina compound tablet

研究了复方苦荞螺旋藻片(BS)对正常及实验性高血糖小鼠血糖水平的影响及其安全性。采用正常及四氧嘧啶建立的高血糖模型小鼠,对BS进行糖耐量和降糖试验研究;采用最大耐受剂量法进行急性经口毒性实验。结果表明,与对照组比较,0.50、1.50g/kg BW的BS对高血糖模型小鼠空腹血糖和糖耐量试验,血糖曲线下面积均有明显的降低作用,降糖率分别达20.05%(P<0.05)和30.79%(P<0.01);血糖曲线下面积分别为25.9(P<0.05)、24.9(P<0.01);对正常小鼠空腹血糖和糖耐量无显著性影响(P>0.05)。BS对正常及模型小鼠的体重和脏体比无统计学意义。安全性实验结果表明,小鼠灌胃最大耐受量大于18.0 g/kg BW。BS对实验性高血糖小鼠具有一定的辅助降血糖作用,对正常小鼠空腹血糖和糖耐量无影响,且无明显毒副作用,使用安全,具有推广应用价值。

The effect of Buckwheat-Spirulina Compound Tablet（ BS） on inhibition of blood sugar level and its security in normal and experimental hyperglycemia mice were investigated. Methods Normal and alloxan-induced hyperglycemia model mice were used,the glucose tolerance and hypoglycemic of BS were tested. The method of Maximum Tolerated Dose（ MTD） was used to carry out the acute oral toxicity test on mice. Results Compared with blank,0. 50,1. 50g / kg BW BS obviously reduced the level of fasting blood-glucose and area under curve of glucose in glucose tolerance experiment of hyperglycemia model mice,the nor-sugar rate were 20. 05%（ P 0. 05） and 30. 79%（ P 0. 01） respectively. The area under curve of glucose were 25. 9（ P 0. 05） and 24. 9（ P 0. 01） respectively.BS had no significant effect on the level of fasting blood-glucose and glucose tolerance experiment of normal mice（ P 0. 05）. BS didn＇t show statistically significant to the avoirdupois and the viscera / body ratio of normal and the model mice. The experiment of safety evaluation showed that the MTD was greater than 18. 0g / kg BW. Conclusion BS had certain assistance to the experimental hyperglycemia mice on the hypoglycemic effect. It didn＇t influenced the level of fasting blood-glucose and glucose tolerance experiment of the normal mice,and had no obvious side effects. Thus it should be safe in application and would be valuable for popularization.