人参皂苷对实验性自身免疫性甲状腺炎大鼠Th1/Th2相关细胞因子的影响

Effect of Ginsenosides on Th1/Th2-Related Cytokines in Rats with Experimental Autoimmune Thyroiditis

目的探讨人参皂苷对实验性自身免疫性甲状腺炎(EAT)大鼠的可能作用机制。方法将40只Wistar大鼠随机分为正常组、模型组和人参皂苷低、中、高剂量组,每组8只,除正常组外其余各组采用猪甲状腺球蛋白(PTg)与弗氏完全佐剂(CFA)和弗式不完全佐剂(IFA)皮下注射6周诱导EAT动物模型的建立。造模后人参皂苷低、中、高剂量组分别给予人参皂苷14mg/kg、28mg/kg、56mg/kg灌胃,每日1次,共8周;正常组及模型组给予等量蒸馏水灌胃。检测各组大鼠血清甲状腺球蛋白抗体(TGAb)、甲状腺过氧化物酶抗体(TPOAb)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)的表达。结果与正常组比较,模型组大鼠TGAb、TPOAb、IL-2水平有明显升高,IL-4水平明显降低(P<0.01);与模型组比较,人参皂苷低、中剂量组均可降低TGAb、TPOAb水平,升高IL-4水平(P<0.05)。结论人参皂苷能降低甲状腺自身抗体,可能通过降低IL-2的表达、升高IL-4的表达而调节Th1/Th2的失衡。

Objective To study the possible mechanism of ginsenosides in regulating Th1/Th2 in rats with experimental autoimmune thyroiditis（EAT）.Methods Forty Wistar rats were randomized into the normal group,model group,low-dose group,middle-dose group,and high-dose group,with 8 in each.The EAT models were established by subcutaneously injecting porcine thyroglobulin（PTg）,Freund＇s complete adjuvant（CFA） and incomplete Freund＇s adjuvant（IFA）for 6 weeks in all groups except the normal group.The low-dose group,middle-dose group and high-dose group was gavaged with 14mg/kg,28mg/kg and 56mg/kg of ginsenosides respectively for 8 weeks.The normal group and model group were given equal amount of distilled water.The expressions of thyroglobulin antibody（TGAb）,thyroid peroxidase antibody（TPOAb）,interleukin-2（IL-2） and IL-4 were detected in all groups.Results Comparing with the normal group,the levels of TGAb,TPOAb and IL-2 were significantly increased but the IL-4 level was significantly decreased in the model group（P〈0.01）.Comparing with the model group,the levels of TGAb and TPOAb were significantly decreased but the IL-4 level was significantly increased in the low-dose group and middle-dose group（P〈0.05）.Conclusion Ginsenosides can reduce thyroid autoantibodies,and its mechanism may be relevant to reducing the expression of IL-2,increasing the expression of IL-4 and regulating the Th1/Th2 imbalance.