脓毒症患者全血中钙结合蛋白S100A12的基因表达水平研究

Study on gene expression of S100A12 protein in whole blood of patients with sepsis

目的检测脓毒症患者全血中钙结合蛋白S100A12的基因表达情况,并将其与炎症反应性疾病的其他炎性指标进行比较,探讨其是否可以作为脓毒症患者诊断的另一种新的指示蛋白。方法实时定量PCR方法检测脓毒症患者44例,以及10例健康人全血中S100A12基因mRNA表达水平,运用2-ΔΔCT算法计算相对表达量,并将S100A12的基因表达水平与白细胞、中性粒细胞绝对值、C反应蛋白、降钙素原水平进行相关性分析。结果在44例脓毒症患者中有15例患者全血中S100A12的基因表达水平升高,高表达率为34.09%;29例病人S100A12的基因表达呈低表达水平,低表达率为65.91%;S100A12的基因表达水平与白细胞、中性粒细胞绝对值、C反应蛋白、降钙素原水平经相关性分析结果显示P值均大于0.05,差异无统计学意义。结论尚不能认为在脓毒症患者全血中S100A12普遍呈高表达状态,暂不能将其作为脓毒症的另一种新的指示蛋白。

Objective To detect the gene expression of S100A12 protein in whole blood of patients with sepsis, and to compare the signif- icance of expression of S100A12 protein with other inflammatory biomarkers, and to explore whether the S100A12 protein can be a faster and relia- ble novel inflammatory indicator of sepsis. Methods The expression of S100A12 gene had been examined by RT - qPCR in 44 patients with sep- sis compared with 10 healthy controls by 2 -aACr method, and the analysis of relationship between S100A12 gene expression with white blood cells （WBC） and neutrophil counts, serum levels of C - reactive protein （CRP） and procalcitonin had been conducted. Results Among 44 patients with sepsis, the expression level of S100A12 protein in 15 patients with sepsis was considered as over-expression, and the percentage of over- expression was 34.09%, and it in 29 patients with sepsis showed low expression and the rate of low - expression was 65.91%. The result of corre- lation analysis showed their difference had no statistical significance （ P 〉 0.05） . Conclusion The expression of S100A12 protein is not very high in patients with sepsis, and it cannot be applied as a novel inflammatory indicator for sepsis at present time.