铂纳米/碳纳米管电化学方法研究及血样中Sal和5-HT的测定

Research on the electroanalytical method for Pt NPs / MWCNTs composite material and the determination of Sal and 5- HT in serum samples

目的：建立测定四氢异喹啉（Sal）和五羟色胺（5-HT） 的电分析化学新方法。方法：首先通过电化学方法制备了铂纳米/碳纳米管复合材料（Pt NPs/MWCNTs） 修饰的玻碳电极，并通过扫描电镜研究了复合材料形貌，同时也研究了Sal和5-HT在Pt NPs/MWCNTs修饰电极上的电化学行为。结果：Sal和5-HT的电化学氧化信号有着明显的分离，对于Sal，峰电流与4.8×10-7~5.2×10-5 mol·L-1呈线性变化（r=0.9995），检测限为1.6×10-7 mol·L-1 （S/N=3）；而对于5-HT，峰电流与5-HT在2.7×10-7~3.07×10-5 mol·L-1范围内呈线性增加（r=0.9993），检测限为9.0×10-8 mol·L-1 （S/N=3）。结论：本方法可以应用于血样中Sal和5-HT的同时灵敏检测，结果令人满意。

Objective： To establish a new electroanalytical method for simultaneous determination of salsolinol （Sal） and 5-hydroxytryptamine （5-HT）in plasma samples. Methods： Pt NPs/MWCNTs composite material was prepared,and its morphology was obtained by scanning electron microscopy.The electrochemical behavior of Sal and 5-HT was investigated at Pt NPs/MWCNTs modified electrode. Results： The experiment result showed the oxidation signals of 5-HT and Sal were obviously separated,and the intensity of the peak current for Sal was linearly related to the concentration in range from 4.8×10-7 to 5.2×10-5 mol·L-1 with a detection limit of 1.6×10-7 mol·L-1 （S/N=3）.The intensity of the peak current for 5-HT was linearly related to the concentration in range from 2.7×10-7 to 3.07×10-5 mol·L-1 with a detection limit of 9.0×10-8 mol·L-1 （S/N=3）. Conclusion： The modified electrode can be used for simultaneous sensitive detection of Sal and 5-HT in serum samples and it has obtained satisfactory results.