硫堇瑞利共振散射荧光法测定肝素钠

Determination of heparin sodium by fluorescence and resonance Rayleigh scattering with thionine

目的：建立硫堇瑞利共振散射荧光法测定肝素钠的含量。方法：在近中性的Britton Robinson（B-R）缓冲溶液中，肝素钠与硫堇形成离子缔合物，在564 nm处生成新的瑞利共振散射（RRS）峰，随着肝素钠浓度的增加RRS峰显著增强。结果：在0.025~0.500 mg·L-1范围内RRS强度与肝素钠浓度呈良好的线性关系，线性回归方程为ΔI=0.125＋16.88C，平行6次测定，RSD为1.67%~3.01%，方法回收率为104.0%~106.4%。结论：硫堇染料作为增色剂的瑞利共振散射荧光法用于肝素钠注射液含量测定结果满意。

Objective： To establish a fluorescence and resonance Rayleigh scattering （RRS） with thionine to determine the content of heparin sodium. Methods： In the near-neutral Britton Robinson （BR） buffer solution,an ion-association complex was formed through the reaction of heparin sodium and thionine,a new RRS spectrum was found at 564 nm,and within a certain range,RRS intensity significantly increased with the increase of heparin sodium concentration. Results： The RRS intensity of the system was linear to the concentration of heparin sodium in the range of 0.025-0.500 mg·L-1 and the linear regression equation was ΔI=0.125＋16.88C.Meanwhile,the relative standard deviations were 1.67%-3.01% for six paralleled determinations,and recoveries were 104.0%-106.4%. Conclusion： The method developed in this paper is applied to the titer analysis of heparin sodium injection and satisfactory results are obtained.