大鼠脊髓损伤的弱激光治疗参数选择

The Optical Parameter Selection of Low-level Laser Therapy in Spinal Cord Injury Rat Models

弱激光在周围神经损伤治疗中取得了理想效果,但在脊髓损伤修复方面的研究很少。本实验应用Allen’s造模法构建大鼠急性脊髓损伤模型,通过测量穿透功率及组织温度变化筛选出用于脊髓损伤治疗的弱激光照射参数,照射组按照筛选出的参数连续治疗14 d,于术后1、3、7、14、21 d应用BBB评分法评价大鼠后肢运动功能的恢复情况,苏木精-伊红染色(HE染色)观察脊髓病理变化并测量空洞面积。结果显示应用筛选出的照射参数(810 nm、光斑面积0.2 cm2、500 mW/cm2、510 J/cm2)连续照射14 d,术后21 d照射组的运动功能评分显著高于未照射组(P<0.05),术后7、14、21d照射组脊髓空洞面积显著小于未照射组(P<0.05)。结果表明810 nm、光斑面积0.2 cm2、500 mW/cm2、510 J/cm2的弱激光照射能促进急性脊髓损伤大鼠后期运动功能的恢复。

Low-level laser therapy （LLLT） has achieved ideal effect on the treatment of peripheral nerve injury,but there was little research in the repair of spinal cord injury （SCI）.Acute spinal cord injury rat model was established by Allen＇ s method.The appropriate parameters of low-level laser therapy was screened by measuring the penetrating power and tissues temperature change.Then low-level laser was applied directly to the lesion site after spinal cord injury for 14 days consecutively according to the result of screening.Subsequently,the hindlimb motor function BBB scales were performed on 1,3,7,14 and 21 day.Hematoxylin-Eosin staining was used to observe spinal cord pathological changes and evaluate the syringomyelia area in both groups.The results showed that,after 21 days of spinal cord injury,the BBB score of laser irradiated group was significantly higher than the control group （P ＜ 0.05） and syringomyelia area in laser irradiated group was also obviously smaller than the control group on 7,14 and 21 day （P ＜0.05）.It turned out that 810 nm,spot area ofO.2 cm2,500 mW/cm2 and 510 J/cm2 low-level laser irradiation can promote motor function recovery safely and effectively after spinal cord injury.