摘要
目的研究中药康肾合剂(KSP)对酒精性肾损害大鼠肾组织血小板衍化生长因子-BB(PDGF-BB)表达的影响。方法 SD雄性大鼠66只,随机分为正常组、酒精组、中药组,每组22只。正'常组给予生理盐水灌胃,酒精组给予乙醇每日2次灌胃,中药组在给予同酒精组等量乙醇基础上,加用康肾合剂水煎剂每日2次灌胃,共12周。于实验第4周、8周、12周末采用免疫组化法检测肾组织PDGF-BB蛋白表达变化。结果酒精组大鼠肾组织PDGF-BB表达显著增强(P<0.05和P<0.01);中药组大鼠PDGF-BB表达明显低于酒精组(P<0.05和P<0.01)。结论大鼠肾组织PDGF-BB表达增强与酒精性肾损害存在一定关联,KSP干预酒精性肾损害的作用可能与其下调PDGF-BB表达有关。
Objective To study the effect of Kangshen Prescription （KSP） on the expression of platelet - derived growth factor （PDGF） -BB in rats alcoholic renal lesion. Methods The animal model of alcoholic renal injury was established with the meth- ods of direct intragastrie infusion of ethanol and KSP. At the end of 4,8 and 12 weeks, PDGF - BB were detected by immunohisto- chemica assay. Results In model group, the expression of PDGF - BB enhanced （ P 〈 O. 05, P 〈 O. O1 ）. Compared with that of model group ,the expression of PDGF- BB was down- regulated （P 〈 0.05, P 〈 0.01 ）. Conclusion PDGF- BB expression en- hancement could be correlated with alcohol - induced nephrotoxieity. By down - regulating the expression of PDGF - BB, KSP could protect rats from alcoholic kidney damage.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2013年第12期2861-2862,共2页
Lishizhen Medicine and Materia Medica Research
基金
甘肃省自然科学基金(No.1107RJZA085)
