秋水仙碱对高脂血症新西兰兔血浆CRP和一氧化氮水平的影响

Effects of Colchicine therapy on plasma level of CRP and nitric oxide in New Zealand rabbits with hyperlipidemia

目的探讨秋水仙碱对高脂血症新西兰兔血浆CRP和一氧化氮水平的影响。方法通过给予高脂饮食构建新西兰兔高脂血症模型,模型构建成功后干预组给予0.01 mg/kg秋水仙碱治疗,共2周。评估空白组、高脂血症对照组和高脂血症干预组3组新西兰兔血脂、CRP及一氧化氮水平。结果经8周高脂饮食饲养后,与空白组相比,高脂血症组新西兰兔血脂指标水平均明显升高,差异有统计学意义(均P<0.05);与空白组相比,血浆CRP水平在高脂血症组也明显升高,而一氧化氮水平则明显降低(P<0.05)。高脂血症干预组与高脂血症对照组血脂水平无明显差异,但高脂血症干预组血浆CRP水平明显降低,而一氧化氮水平则明显升高,与高脂血症对照组比较差异有统计学意义(均P<0.05)。结论秋水仙碱能够通过下调血浆CRP水平从而改善高脂血症新西兰兔血管内皮细胞功能,这可能是秋水仙碱能够减少冠心病患者心血管事件的其中一个作用机制。

Objective To investigate the effects of Colchicine therapy on plasma level of CRP and （nitric oxide） NO in New Zealand rabbits with hyperlipidemia. Methods Hyperlipidemia model was induced by institution of high cholesterol diet in New Zealand rabbits,and colchicines （0.01 mg/kg body weight）was administered for 2 weeks when hyperlipidemia model was established. Plasma level of lipid profile,CRP and NO were evaluated in blank group,hyperlipidemia control group and hyperlipidemia intervention group. Re-sults With 8 weeks high cholesterol diet treatment,plasma level of lipid profile was significantly higher when compared to blank group;and the plasma level of CRP was also higher while NO was lower in hyperlipidemia groups. There was no significant difference of lipid profile in hyperlipidemia control group and hyperlipidemia intervention group after 2 weeks colchicine therapy,however the plasma level of CRP was significant lower while NO was significant higher in the hyperlipidemia intervention group when compared to the hyperlipidemia control group. Conclusion Endothelial functions was improved by colchicine therapy in hyperlipidemia rab-bit,which may be ascribed to the down-regulation of CRP,and this may be one of the potential mechanisms colchicine reduce cardiovascular events in patients with coronary artery diseases.