p53基因突变与人非小细胞肺癌临床病理生理分析

Analysis of the relationship between p53 gene mutation and clinical pathophysiological characteristics of primary non small cell lung cancer

目的 探讨p5 3基因点突变与人非小细胞肺癌临床病理生理特征的联系。方法 应用聚合酶反应 -单链构象多态性分析方法 (PCR SSCP)检测原发性非小细胞肺癌癌组织的p5 3基因第 5～ 8外显子点突变。结果 40例肺癌组织中 19例 ( 4 7.5 % )有点突变发生 ,8例良性肿瘤组织均无p5 3基因点突变发生。点突变发生与病理分期和淋巴结转移有明显关系 (P <0 .0 2 5 )。结论 p5 3基因点突变在非小细胞肺癌的发生和进展中可能起重要作用。

Objective To investigate the significance of p53 gene mutations in non small cell lung cancer, with focusing on the four highly conserved regions of the p53 gene. Methods Point mutations of the p53 gene were detected in 40 lung cancer tissues and 8 benign pulmonary lesions as control by polymerase chain reaction single strand conformation polymorphism (PCR SSCP) analysis. Results Mutations of the p53 gene were found in 19 of 40 lung cancer samples ( 47.5%) . The frequency of the p53 gene mutations varied among the different stage (stage Ⅰ+Ⅱ vs stage Ⅲ+Ⅳ was 22.2% vs 68.2%,P<0.005), and lymph node netastasis status (N1 2 vs N0 was 57.1% vs 16.7%, P<0.025) of non small cell lung cancers. Conclusion The results suggest that p53 gene mutations may be associated with the carcinogenesis and progression of non small cell lung cancer.