外源性雌激素及二仙汤对雷公藤多苷诱导大鼠卵巢早衰防治作用的比较研究

Comparative Study of Preventive and Therapeutic Effects Between Xenoestrogen and “Erxian Decoction”on Premature Ovarian Failure Induced by Tripterygium Glycosides in Rats

目的:比较外源性雌激素和二仙汤对雷公藤多苷诱导的卵巢早衰的防治作用。方法:将雌性SD大鼠随机分为空白对照组、模型组、雌激素组和二仙汤组。应用雷公藤多苷制备卵巢早衰大鼠病理模型,空白组与模型组均灌胃给予生理盐水,雌激素组和二仙汤组分别灌胃给予雌激素和二仙汤。干预21 d后,检测大鼠子宫、卵巢和肾上腺脏器系数,应用放免法检测血清雌激素(E2)、黄体生成素(LH)和卵泡刺激素(FSH)的水平。结果:外源性雌激素能够显著增加子宫、卵巢和肾上腺脏器系数(P<0.05,P<0.01),能够恢复卵巢早衰大鼠E2水平(P<0.05),而LH和FSH无明显变化;二仙汤组大鼠卵巢和肾上腺脏器系数显著增加(P<0.05,P<0.01),子宫脏器系数和血清性激素水平无明显变化。结论:外源性雌激素能够改善雷公藤多苷片诱导的卵巢早衰,疗效优于二仙汤。

Objective ： To compare the preventive and therapeutic effects of exogenous estrogen and ＂Erxian Decoction＂ on premature overian failure （POF） induced by tripterygium glycosides in rats. Methods： Female SD rats were randomized into blank control group, model group, estrogen group and ＂Erxian Decoction＂ group. The models were established by tripteryglum glycosides. The rats in blank control group and model group were treated with normal saline intragastrically, and rats in estrogen group and ＂Erxian Decoction＂ group were treated with estrogen and ＂Erxian Decoction＂ respectively intragastrically. After 21 days intervention, the coefficient of uterus, ovary and adrenal glands were detected and the levels of E2 , LH and FSH were detected by radioimmunoassay. Results： Exogenous estrogen can increase organ indexes of uterus, ovary and adrenal glands （P 〈0.05, P 〈0.01 ） , and also can recover the E2 level in rat with POF, but no significant changes was found in LH and FSH; the organ indexes of ovary and adrenal glands were increased in ＂Erxian Decoction＂ group （P 〈 0.05, P 〈 0. 01 ） , and no significant difference was found in uterus index and serum sexual hormone level. Conclusion： Exogenous estrogen has positive effects on POF, and its therapeutic effect is better than ＂Erxian Decoction＂.