KCNN3基因多态性与非瓣膜病房颤的关联研究

Association Study of SNP in KCNN3 with Non-valvular Atrial Fibrillation

目的探讨房颤与小电导Ca^(2+)激活K^+通道基因KCNN3的部分基因多态性关系。方法选取2009年1月至2013年6月就诊于我院的非瓣膜病房颤患者(房颤组)及匹配的对照患者(对照组)各123例。提取外周血白细胞基因组DNA,PCR扩增KCNN3目的序列,对其标签SNP rs11584403位点多态性进行测序分析。结果 KCNN3基因rs11584403位点多态性在房颤组与对照组间有统计学差异(P<0.01);分别在共显性、显性、隐形遗传模式下,少数等位基因G携带者发生房颤的风险是多数等位基因T携带者的3.11倍、1.75倍及2.37倍。结论 KCNN3基因rs11584403位点的G等位基因可能增加房颤的患病风险,KCNN3基因可能是房颤的易感基因。

Objective To investigate whether rs11584403 in KCNN3 correlates with non-valvular atrial fibrillation （AF） in Chinese. Methods Rs11584403 pulymorphisms were screened by direct DNA sequencing of KCNN3 in 123 AF patients and 123 controls of Chinese Han population. Results There were significant differences between the AF cases and controls in beth genotype distribution and allele fxequencies of SNP rs11584403. Logistic regression revealed that rs11584403 in KCNN3 is highly associated with AF. The difference of frequencies of genotype and al- leles between the two groups were statistically significant. Compared to allele T, allele G significantly increased the risk of AF. The odds ratios （OR） were 3.11 （ 1.44- 6.70）, 1.75 （ 1.05-2.89 ） and 2.73 （ 1.31 -5.66 ） under the codominant model, dominant model, and recessive model, respectively. Conclusion The G polymorphism in rs11584403 of KCNN3 gene is associated with AF in Han Chinese population. KCNN3 may be the predispos- ing factor of non-valvallar AF.