钙离子在VP-16诱导HL-60细胞凋亡的作用

The Role of Calcium Ion in Apotopsis of HL-60 Cells Induced by VP-16

为了研究Ca2 + 在VP 16(etoposide)诱导HL 60细胞凋亡中的作用 ,采用流式细胞术 ,激光共聚焦显微镜和Western印迹方法进行观察。结果显示 ,VP 16可诱导HL 60细胞凋亡 ,并可引起细胞内Ca2 + 浓度的增加 ;细胞外钙离子螯合剂EGTA不抑制其诱导的细胞凋亡 ,而细胞内钙离子螯合剂BAPTA AM可抑制此过程 ,并且可抑制细胞色素C的释放。实验结果提示 ,Ca2 +

To study the significance of calcium in the apoptosis of HL-60 cells induced by VP-16, the technology of flow cytometry, confocal laser scanning microscopy and Western blot were used. The results showed that VP-16 could induced the apoptosis of HL-60 cells and transient increase of intracellular calcium concentration; EGTA〔ethylene glycol-bis(2-aminoethyl)-N,N,N′,N′-tetraacetic acid〕, that could combine the extracellular calcium, did not prevent the apoptosis of HL-60 cells. BAPTA-AM〔1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxy-methyl)ester〕, however, a chelating agent of intracellular calcium ions, could prevent apoptosis and the release of cytochrome C from HL-60 cells. It was concluded that the calcium plays an important role in apoptosis and the release of cytochrome C.