UPLC-MS/MS法测定牛奶中链霉素和双氢链霉素残留

Detection of streptomycin and dihydrostreptomycin residues in milk by UPLC- MS/MS

建立了牛奶中链霉素和双氢链霉素的超高效液相色谱-串联质谱法（UPLC—MS／MS）。牛奶中的目标物经磷酸溶液提取后，分别通过苯磺酸型和羧酸型固相萃取柱净化、浓缩，再用超高效液相色谱-串联质谱仪进行测定，外标法定量。确定了链霉素和双氢链霉素的线性回归方程；方法的回收率分别为77．4％-98．2％和80．1％-95．2％，且相对标准偏差分别为5．4％-7．2％和6．4％-8．6％。该方法快速、准确，回收率高，可用于牛奶中链霉素和双氢链霉素的同时检测。

The paper establishes a method to determine streptomycin and dihydrostreptomycin contents in milk using UPLC-MS/MS. Phosphoric acid solution was added to milk samples to extract target ingredient, and then concentrated by SCX and WCX SPE procedures successively. Streptomycin and dihydrostrept0mycin contents were measured by UPLC-MS/MS. The recoveries of each compounds were 77.4%-98.2% and 80.1%-95.2%, and the relative standard deviations are 5.4%-7.2% and 6.4%-8.6% for streptomycin and dihydrostreptomycin respectively, which showsed that the method meet the demand to determine streptomycin and dihydrostreptomycin contents in milk.