D-氨基酸氧化酶结构的研究进展

Research Progress on the Crystal Structure of D-amino Acid Oxidase

D-氨基酸氧化酶氧化D-氨基酸生成相应的α-酮酸和氨,还原型黄素被氧化生成过氧化氢。D-氨基酸氧化酶在精神分裂症的病理、生理学研究和D-氨基酸检测等方面都发挥着关键的作用。主要就结构已解析的几个D-氨基酸氧化酶的序列同源性、结构组成、活性中心、抑制剂以及与辅酶FAD结合能力等方面进行介绍。

D-amino acid oxidase catalyzes oxidative deamination of D-amino acids yielding α-keto acid and ammonia, the reduced flavin is reoxidized to generate hydrogen peroxide by molecular oxygen. D-amino acid oxidase plays a key role in the pathophysiology of schizophrenia research and D-amino acids detecting. Based on the three-dimensional structure of analyzed D-amino acid oxidases, the property of the enzyme could be improved by rational protein design. In order to get a better understanding of D-amino acid oxidase, the sequence homology, overall structure, the active site center, inhibitor, FAD binding site and flexible parts of DAAO were introduced.