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大豆乙烯响应因子GmERF6的功能分析 被引量：3

Functional Analysis of Ethylene-response Factor GmERF6 in Soybean
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摘要 GmERF6是从大豆中克隆到的一个新的ERF转录抑制子基因,对其功能作了进一步的分析。亚细胞定位试验结果显示GmERF6蛋白定位于细胞核中;利用原核表达纯化GmERF6蛋白并进行凝胶阻滞分析(EMSA),结果显示,GmERF6蛋白在体外可以与GCC-box元件特异结合;对GmERF6转基因拟南芥干旱条件下的种子萌发率和植株表型进行观察,结果证实GmERF6提高了转基因拟南芥的抗旱性,表明该基因可作为培育抗旱材料的候选基因。 GmERF6 is a new ERF transcription repressor isolated from soybean. The function of GmERF6 was further analyzed in this research. GmERF6 protein localized to the nucleus when transiently expressed in onion epidermal cells. Furthermore, EMSA showed GmERF6 protein binding to the GCC-box element in vitro . The seed germination rates of GmERF6 transgenic Arabidopsis were significantly higher than that of wild type Arabidopsis under drought stress. GmERF6 transgenic Arabidopsis grew much healthier than wild-type Arabidopsis by removing water for 18 d. These data indicated that GmERF6 enhanced drought tolerance in transgenic Arabidopsis . These results suggest GmERF6 is likely to be a useful tool for improving drought tolerance of plants.
作者 翟莹 赵艳 杨晓杰 孙天国 张军 姚雅男
机构地区 齐齐哈尔大学生命科学与农林学院遗传重点实验室 黑龙江省兽医科学研究所 长春农业博览园
出处 《生物技术通报》 CAS CSCD 北大核心 2013年第12期73-77,共5页 Biotechnology Bulletin
基金 齐齐哈尔大学青年教师科研启动支持计划项目(2012k-M20)
关键词 大豆 GmERF6 亚细胞定位 EMSA 抗旱性 Soybean GmERF6 Subcellular localization EMSA Drought tolerance
分类号 S565.1 [农业科学—作物学]
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参考文献24

  • 1Xiong L, Ishitani M, Zhu JK. Interaction of osmotic stress, temperature and abscisic acid in the regulation of gene expression in Arabidopsis [J]. Plant Physiol, 1999, 119(1):205-211.
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二级参考文献23

  • 1葛艳辉,崔继哲,于丽杰,宋文华.影响农杆菌介导白细胞介素-2基因转化番茄的因素研究[J].植物研究,2006,26(4):470-474. 被引量：4
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生物技术通报
2013年 第12期

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